General Superoxide Dismutase (SOD) Assay Kit | SOD assay kit
Superoxide Dismutase (SOD) Typed Activity Assay Kit (Hydroxylamine Method)
Reactivity
General
Synonyms
Superoxide Dismutase (SOD); N/A; Superoxide Dismutase (SOD) Typed Activity Assay Kit (Hydroxylamine Method); SOD assay kit
Reactivity
General
Specificity
96T (Can detect 92 samples without duplication)
Samples
Serum, plasma, urine, cells, cell culture supernatant and tissue homogenate samples
Detection Range
1.35-62 U/mL
Sensitivity
1.35 U/mL
Intra-assay Precision
The intra-assay CV is 5.1%.
Inter-assay Precision
The inter-assay CV is 9.6%.
Preparation and Storage
Reagent4: -20 degree C; Others: 2-8 degree C. Valid for 12 months.
Related Product Information for SOD assay kit
Background: According to the literature, superoxide dismutase exists in all oxygenmetabolizing cells to protect cells from excessive superoxide. Under the action of SOD, two superoxide anions were converted to oxygen and hydrogen peroxide.In mammals, there are three different forms of SOD: CuZn-SOD, Mn-SOD and EC-SOD (an extracellular form of SOD). Cu-Zn SOD exists in the cytoplasmic and mitochondrial membrane spaces of the cells, while Mn-SOD is located in the mitochondrial matrix.
Principle of the Assay: Superoxide anion (O2•-) produced by xanthine and xanthine oxidase system can oxidize hydroxylamine to form nitrite which appear purplish red after chromogenic reaction. The SOD in the sample has a specific inhibitory effect on superoxide anion (O2•-), can reduce the content of nitrite. The OD value is lower than control and the activity of SOD is calculated through the formula. There are two kinds of SOD (CuZn-SOD, Mn-SOD) in the cells of higher animals, and the sum of them is equal to the total SOD. The activity of Mn-SOD in the sample will lost after sample pretreatment, but the activity of CuZn-SOD will not.
Intended Uses: This kit can be used to measure T-SOD, CuZn-SOD, Mn-SOD activity in serum, plasma, urine, cells, cell culture supernatant and tissue homogenate samples.
Principle of the Assay: Superoxide anion (O2•-) produced by xanthine and xanthine oxidase system can oxidize hydroxylamine to form nitrite which appear purplish red after chromogenic reaction. The SOD in the sample has a specific inhibitory effect on superoxide anion (O2•-), can reduce the content of nitrite. The OD value is lower than control and the activity of SOD is calculated through the formula. There are two kinds of SOD (CuZn-SOD, Mn-SOD) in the cells of higher animals, and the sum of them is equal to the total SOD. The activity of Mn-SOD in the sample will lost after sample pretreatment, but the activity of CuZn-SOD will not.
Intended Uses: This kit can be used to measure T-SOD, CuZn-SOD, Mn-SOD activity in serum, plasma, urine, cells, cell culture supernatant and tissue homogenate samples.
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Product Notes
The General SOD (Catalog #AAA176972) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA176972 Assay Kit recognizes General SOD. It is sometimes possible for the material contained within the vial of "Superoxide Dismutase (SOD), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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