General PD1 (Protectin D1) ELISA Kit | PD1 elisa kit
Universal PD1 (Protectin D1) ELISA Kit
Reactivity
General
Synonyms
PD1 (Protectin D1); N/A; Universal PD1 (Protectin D1) ELISA Kit; PD1 elisa kit
Reactivity
General
Specificity
Specifically recognize PD1, no obvious cross reaction with other analogues
Assay Type
Competitive
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
0.156-10ng/ml
Sensitivity
0.094ng/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Related Product Information for PD1 elisa kit
Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with PD1. During the reaction, PD1 in the sample or standard competes with a fixed amount of PD1 on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to PD1. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of PD1 in the samples is then determined by comparing the OD of the samples to the standard curve. The concentration of the target substance was inversely proportional to the OD450 value.
