General PEA (Pseudomonas Exotoxin A) ELISA Kit | PEA elisa kit

PEA (Pseudomonas Exotoxin A) ELISA Kit

Cat. Num. AAA249873

• Reactivity: General
• Synonyms: PEA (Pseudomonas Exotoxin A); N/A; PEA (Pseudomonas Exotoxin A) ELISA Kit; Exotoxin A; ETA; NAD(+)--diphthamide ADP-ribosyltransferase; PE; eta; PA1148; PEA; Pseudomonas Exotoxin A; PEA elisa kit

• Reactivity: General
• Specificity: Specifically recognize PEA, no obvious cross reaction with other analogues
• Assay Type: Sandwich
• Samples: Serum, plasma, cell culture supernatant and other biological samples
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml

• Intra-assay Precision: Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
• Inter-assay Precision: Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Related Product Information for PEA elisa kit

Background: Pseudomonas Exotoxin A (PE) is a bacterial exotoxin produced by Pseudomonas aeruginosa. This toxin is highly toxic and can cause cell death and tissue damage. PE is a single-chain protein with three functional domains: a cytotoxic domain, a cell membrane-binding domain, and a translocation domain. The cytotoxic domain contains a sequence with ADP-ribosylation activity, which can enter cells and inhibit protein synthesis. The cell membrane-binding domain can bind to certain molecules on the cell surface, facilitating the entry of PE into the cell. The translocation domain helps PE enter the endoplasmic reticulum inside the cell and release its toxin activity. PE mainly causes disease through bacterial infection, such as cancer and AIDS.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti PEA antibody was pre-coated onto the 96-well plate. The biotin conjugated anti PEA antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with PEA conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of PEA in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

NCBI and Uniprot Product Information

• NCBI GI #: 15596345
• NCBI GeneID: 877850
• NCBI Accession #: NP_249839.1
• NCBI GenBank Nucleotide #: NC_002516.2
• UniProt Accession #: P11439; Q9I4I7
• Molecular Weight: 69,284 Da
• NCBI Official Full Name: exotoxin A
• NCBI Official Symbol: toxA
• NCBI Protein Information: exotoxin A
• UniProt Protein Name: Exotoxin A
• UniProt Gene Name: eta
• UniProt Synonym Gene Names: ETA