General PGE1 ELISA Kit | PGE1 elisa kit

PGE1 (Prostaglandin E1) ELISA Kit

Cat. Num. AAA164417

• Gene Names: P2RY12; HORK3; P2Y12; ADPG-R; BDPLT8; SP1999; P2T(AC); P2Y(AC); P2Y(12)R; P2Y(ADP); P2Y(cyc)
• Reactivity: General
• Synonyms: PGE1; N/A; PGE1 (Prostaglandin E1) ELISA Kit; PGE1 elisa kit

• Reactivity: General
• Specificity: This kit recognizes PGE1 in samples. No significant cross-reactivity or interference between PGE1 and analogues was observed.
• Sequence Length: 342
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma and other biological fluids
• Detection Range: 78.13-5000pg/mL
• Sensitivity: 46.88pg/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level PGE1 were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level PGE1 were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Standard Curve (Sample)

Related Product Information for PGE1 elisa kit

Principle of the Assay: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with PGE1. During the reaction, PGE1 in the sample or standard competes with a fixed amount of PGE1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to PGE1. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PGE1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of PGE1 concentrations in serum, plasma and other biological fluids.

NCBI and Uniprot Product Information

• NCBI GI #: 12232483
• NCBI GeneID: 64805
• NCBI Accession #: NP_073625.1
• NCBI GenBank Nucleotide #: NM_022788.4
• UniProt Accession #: Q9H244; Q546J7; D3DNJ5
• Molecular Weight: 39,439 Da
• NCBI Official Full Name: P2Y purinoceptor 12
• NCBI Official Synonym Full Names: purinergic receptor P2Y, G-protein coupled, 12
• NCBI Official Symbol: P2RY12
• NCBI Official Synonym Symbols: HORK3; P2Y12; ADPG-R; BDPLT8; SP1999; P2T(AC); P2Y(AC); P2Y(12)R; P2Y(ADP); P2Y(cyc)
• NCBI Protein Information: P2Y purinoceptor 12; ADP-glucose receptor; G-protein coupled receptor SP1999; Gi-coupled ADP receptor HORK3; P2Y12 platelet ADP receptor; purinergic receptor P2RY12; putative G-protein coupled receptor
• UniProt Protein Name: P2Y purinoceptor 12
• UniProt Gene Name: P2RY12
• UniProt Synonym Gene Names: HORK3; P2Y12; ADPG-R; P2Y(ADP)
• UniProt Entry Name: P2Y12_HUMAN