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product-image-AAA76242_AD10.jpg Application Data (Biotinylated Human GFRAL/GFR alpha-like Protein Blocking DataSerial dilutions of Anti-GDF15 Antibody were added into Human GDF15, His Tag : Biotinylated Human GFRAL, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 40.8ng/ml.)

GFRAL/GFR alpha-like Recombinant Protein | GFRAL recombinant protein

Biotinylated Mouse GFRAL/GFR alpha-like Protein Recombinant

Gene Names
Gfral; Gral; AY457637
Purity
>95%, as determined by SDS-PAGE and HPLC
Synonyms
GFRAL/GFR alpha-like; N/A; Biotinylated Mouse GFRAL/GFR alpha-like Protein Recombinant; GFRAL recombinant protein
Ordering
Host
HEK293 cells (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.)
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant Mouse GFRAL/GFR alpha-like was lyophilized from 0.2 um filtered PBS solution pH7.4
Source
DNA sequence encoding Mouse GFRAL/GFR alpha-like including a N-His-Avi tag was expressed in HEK293 Cells.The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at-20 degree C.

Application Data

(Biotinylated Human GFRAL/GFR alpha-like Protein Blocking DataSerial dilutions of Anti-GDF15 Antibody were added into Human GDF15, His Tag : Biotinylated Human GFRAL, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 40.8ng/ml.)

product-image-AAA76242_AD10.jpg Application Data (Biotinylated Human GFRAL/GFR alpha-like Protein Blocking DataSerial dilutions of Anti-GDF15 Antibody were added into Human GDF15, His Tag : Biotinylated Human GFRAL, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 40.8ng/ml.)

ELISA

(Biotinylated Human GFRAL/GFR alpha-like Protein ELISA DataImmobilized Human GDF15, His Tag at 1?g/ml (100?l/well) on the plate. Dose response curve for Biotinylated Human GFRAL, His Tag with the EC50 of 0.1?g/ml determined by ELISA.)

product-image-AAA76242_ELISA11.jpg ELISA (Biotinylated Human GFRAL/GFR alpha-like Protein ELISA DataImmobilized Human GDF15, His Tag at 1?g/ml (100?l/well) on the plate. Dose response curve for Biotinylated Human GFRAL, His Tag with the EC50 of 0.1?g/ml determined by ELISA.)

Application Data

(Biotinylated Human GFRAL/GFR alpha-like Protein SEC-HPLCThe purity of Biotinylated Human GFRAL is greater than 95% as determined by SEC-HPLC.)

product-image-AAA76242_AD13.jpg Application Data (Biotinylated Human GFRAL/GFR alpha-like Protein SEC-HPLCThe purity of Biotinylated Human GFRAL is greater than 95% as determined by SEC-HPLC.)

Tris-Bis-PAGE

(Biotinylated Human GFRAL/GFR alpha-like Protein Tris-Bis PAGEBiotinylated Human GFRAL on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.)

product-image-AAA76242_PAGE15.jpg Tris-Bis-PAGE (Biotinylated Human GFRAL/GFR alpha-like Protein Tris-Bis PAGEBiotinylated Human GFRAL on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.)
Product Categories/Family for GFRAL recombinant protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
43,964 Da
NCBI Official Full Name
GDNF family receptor alpha-like
NCBI Official Synonym Full Names
GDNF family receptor alpha like
NCBI Official Symbol
Gfral
NCBI Official Synonym Symbols
Gral; AY457637
NCBI Protein Information
GDNF family receptor alpha-like; GDNF receptor alpha-like
UniProt Protein Name
GDNF family receptor alpha-like
UniProt Gene Name
Gfral
UniProt Synonym Gene Names
Gral
UniProt Entry Name
GFRAL_MOUSE

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Product Notes

The GFRAL gfral (Catalog #AAA76242) is a Recombinant Protein produced from HEK293 cells (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.) and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "GFRAL/GFR alpha-like, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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