Standard Curve (Sample)
Rat ghrelin/obestatin prepropeptide ELISA Kit | GHRL elisa kit
Rat ghrelin, GHRL ELISA Kit
Reactivity
Rat
Synonyms
ghrelin/obestatin prepropeptide; N/A; Rat ghrelin, GHRL ELISA Kit; Rat ghrelin (GHRL) ELISA kit; MTLRP; obestatin; OTTHUMP00000207794; OTTHUMP00000207795; OTTHUMP00000207796; OTTHUMP00000207798; ghrelin; growth hormone secretagogue receptor ligand; ghrelin/obestatin; ghrelin/obestatin preprohormone; m; GHRL elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat GHRL. No significant cross-reactivity or interference between Rat GHRL and analogues was observed.
Sequence Length
117
Samples
Serum, plasma, cell culture supernates
Detection Range
7.8 pg/ml - 500 pg/ml
Sensitivity
1.95 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for GHRL elisa kit
Introduction: Ghrelin was originally discovered as a preprohormone that stimulates the release of growth hormone from the anterior pituitary. Recent studies have shown that ghrelin, in concert with other hormones, helps to control both appetite and energy balance. The synthesis of ghrelin takes place predominantly in epithelial cells of the stomach, although it is also produced in smaller quantities in the placenta, kidney, pituitary and hypothalamus. Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor and is involved in regulating growth hormone release from the pituitary. It has an appetite stimulating effect, induces adiposity, and stimulates gastric acid secretion, and it is involved in growth regulation. Ghrelin is synthesized as a preprohormone (13kDa), then proteolytically processed to yield a 28 amino acid peptide (3kDa). Obestatin, an appetite suppressing peptide hormone, is also produced in the process. An interesting and unique modification is imposed on the ghrelin hormone during synthesis in the form of octanoic acid bound to one of its amino acids. This modification is necessary for biological activity.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GHRP-Ghrelin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GHRP-Ghrelin. and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain GHRP-Ghrelin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of GHRP-Ghrelin. in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GHRP-Ghrelin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GHRP-Ghrelin. and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain GHRP-Ghrelin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of GHRP-Ghrelin. in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13,176 Da
NCBI Official Full Name
appetite-regulating hormone preproprotein
NCBI Official Synonym Full Names
ghrelin/obestatin prepropeptide
NCBI Official Symbol
Ghrl
NCBI Protein Information
appetite-regulating hormone; ghrelin; motilin-related peptide; growth hormone secretagogue; growth hormone-releasing peptide
UniProt Protein Name
Appetite-regulating hormone
UniProt Gene Name
Ghrl
UniProt Entry Name
GHRL_RAT
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Product Notes
The Rat GHRL ghrl (Catalog #AAA80424) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA80424 ELISA Kit recognizes Rat GHRL. It is sometimes possible for the material contained within the vial of "ghrelin/obestatin prepropeptide, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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