Mouse Glucose Transporter 2 ELISA Kit | GLUT2 elisa kit

Mouse Glucose Transporter 2 ELISA Kit

Cat. Num. AAA16424

• Gene Names: Slc2a2; Glut2; Glut-2; AI266973
• Reactivity: Mouse
• Synonyms: Glucose Transporter 2; N/A; Mouse Glucose Transporter 2 ELISA Kit; GLUT2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of GLUT2. No significant cross-reactivity or interference between GLUT2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GLUT2 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive or Sandwich
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for GLUT2 elisa kit

Principle of the Assay: GLUT2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-GLUT2 antibody and an GLUT2-HRP conjugate. The assay sample and buffer are incubated together with GLUT2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GLUT2 concentration since GLUT2 from samples and GLUT2-HRP conjugate compete for the anti-GLUT2 antibody binding site. Since the number of sites is limited, as more sites are occupied by GLUT2 from the sample, fewer sites are left to bind GLUT2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GLUT2 concentration in each sample is interpolated from this standard curve.

Intended Uses: This GLUT2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse GLUT2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for GLUT2 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 683589
• NCBI GeneID: 20526
• UniProt Accession #: P14246; Q3TNA5; Q9DBA7
• Molecular Weight: 57,107 Da
• NCBI Official Full Name: GLUT2, partial
• NCBI Official Synonym Full Names: solute carrier family 2 (facilitated glucose transporter), member 2
• NCBI Official Symbol: Slc2a2
• NCBI Official Synonym Symbols: Glut2; Glut-2; AI266973
• NCBI Protein Information: solute carrier family 2, facilitated glucose transporter member 2; liver-type glucose transporter; glucose transporter type 2, liver
• UniProt Protein Name: Solute carrier family 2, facilitated glucose transporter member 2
• UniProt Gene Name: Slc2a2
• UniProt Synonym Gene Names: Glut-2; Glut2; GLUT-2
• UniProt Entry Name: GTR2_MOUSE