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product-image-AAA79665_SC15.png Standard Curve (Sample)

Rat gonadotropin-releasing hormone 1 (luteinizing-releasing hormone) ELISA Kit | GNRH1 elisa kit

Rat gonadotropin-releasing hormone, GnRH ELISA Kit

Gene Names
Gnrh1; Gnrh; Lhrh; Gnrha; Rgnrhg1
Reactivity
Rat
Synonyms
gonadotropin-releasing hormone 1 (luteinizing-releasing hormone); N/A; Rat gonadotropin-releasing hormone, GnRH ELISA Kit; Rat gonadotropin-releasing hormone; GnRH ELISA Kit; GNRH; GRH; LHRH; LNRH; gonadotropin-releasing hormone 1; gonadotropin-releasing hormone 1 (leutinizing-releasing hormone) ; gonadotropin-releasing hormone 1 (lutienizing hormone-releasing hormone) ; GNRH1 elisa kit
Ordering
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of rat GnRH. No significant cross-reactivity or interference between rat GnRH and analogues was observed.
Sequence Length
92
Samples
Serum, plasma, tissue homogenates
Assay Type
Competitive
Detection Range
2 pg/ml -800 pg/ml.
Sensitivity
The minimum detectable dose of rat GnRH is typically less than 2pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest rat GnRH concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

product-image-AAA79665_SC15.png Standard Curve (Sample)
Related Product Information for GNRH1 elisa kit
Principle of the assay: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for GnRH and Horseradish Peroxidase (HRP) conjugated GnRH. The competitive inhibition reaction is launched between with HRP labeled GnRH and unlabeled GnRH with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of GnRH in the sample. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
10,500 Da
NCBI Official Full Name
progonadoliberin-1
NCBI Official Synonym Full Names
gonadotropin-releasing hormone 1 (luteinizing-releasing hormone)
NCBI Official Symbol
Gnrh1
NCBI Official Synonym Symbols
Gnrh; Lhrh; Gnrha; Rgnrhg1
NCBI Protein Information
progonadoliberin-1; progonadoliberin I; gonadotropin releasing hormone 1; luteinizing hormone-releasing hormone
UniProt Protein Name
Progonadoliberin-1
UniProt Gene Name
Gnrh1
UniProt Synonym Gene Names
Gnrh; GnRH-I; LH-RH I
UniProt Entry Name
GON1_RAT

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Product Notes

The Rat GNRH1 gnrh1 (Catalog #AAA79665) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA79665 ELISA Kit recognizes Rat GNRH1. It is sometimes possible for the material contained within the vial of "gonadotropin-releasing hormone 1 (luteinizing-releasing hormone), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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