Goat OCIL Antibody | anti-OCIL antibody

Goat Anti-OCIL Antibody

Cat. Num. AAA61831

• Gene Names: CLEC2D; CLAX; LLT1; OCIL
• Reactivity: Human; Expected from sequence similarity: Human, Dog, Cow
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: OCIL; N/A; Goat Anti-OCIL Antibody; CLEC2D; C-type lectin domain family 2, member D; CLAX; LLT1; C-type lectin related f; C-type lectin superfamily 2, member D; lectin-like NK cell receptor; lectin-like transcript 1; osteoclast inhibitory lectin; anti-OCIL antibody

• Host: Goat
• Reactivity: Human; Expected from sequence similarity: Human, Dog, Cow
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Sequence Length: 191

• Applicable Applications for anti-OCIL antibody: IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), IF (Immunofluorescence), WB (Western Blot), ELISA (Peptide ELISA)
• Immunogen: Peptide with sequence C-LLRYKGPSDH, from the Internal region of the protein sequence according to NP_037401.1; NP_001004419.1; NP_001184246.1.
• Peptide Sequence: LLRYKGPSDH
• Epitope: Internal region
• Important comments: This antibody is expected to recognize all isoforms (NP_037401.1; NP_001004419.1; NP_001184246.1).
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

IHC (Immunohistochemistry)

((3.75ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

IHC (Immunohistochemistry)

((3.75ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) f)

IF (Immunofluorescence)

(Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic/membrane staining. The nuclear stain i)

WB (Western Blot)

((0.3ug/ml) staining of U937 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)

References

Skinningsrud B, Husebye ES, Pearce SH, McDonald DO, Brandal K, Wolff AB, Løvås K, Egeland T, Undlien DE.: Polymorphisms in CLEC16A and CIITA at 16p13 are associated with primary adrenal insufficiency. J Clin Endocrinol Metab. 2008 Sep;93(9):3310-7. Epub 2008 Jul 1. PMID: 18593762

NCBI and Uniprot Product Information

• NCBI GI #: 7019447
• NCBI GeneID: 29121
• NCBI Accession #: NP_037401.1; NP_001004419.1; NP_001184246.1
• NCBI GenBank Nucleotide #: NP_037401.1
• UniProt Accession #: Q9UHP7
• NCBI Official Full Name: C-type lectin domain family 2 member D isoform 1
• NCBI Official Synonym Full Names: C-type lectin domain family 2 member D
• NCBI Official Symbol: CLEC2D
• NCBI Official Synonym Symbols: CLAX; LLT1; OCIL
• NCBI Protein Information: C-type lectin domain family 2 member D
• UniProt Protein Name: C-type lectin domain family 2 member D
• UniProt Gene Name: CLEC2D
• UniProt Synonym Gene Names: CLAX; LLT1; OCIL; LLT-1