Goat c-FOS Antibody | anti-FOS antibody

Goat anti-c-FOS (aa283-295) Antibody

Cat. Num. AAA13673

• Gene Names: FOS; p55; AP-1; C-FOS
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Dog, Pig, Cow
• Applications: ELISA, Western Blot, Immunofluorescence, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: c-FOS; N/A; Goat anti-c-FOS (aa283-295) Antibody; activator protein 1, AP-1, cellular oncogene c-fos, C-FOS, FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS), FBJ murine osteosarcoma viral oncogene homolog, FOS, G0/G1 switch regulatory protein 7, p55, proto-oncogene c-Fos; anti-FOS antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Dog, Pig, Cow
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Sequence Length: 380

• Applicable Applications for anti-FOS antibody: Peptide ELISA (EIA), Western Blot (WB), Immunofluroescence (IF), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 128000.; IHC: Paraffin embedded Human Tonsil. Recommended concentration: 8ug/ml; Immunofluorescence : Strong expression of the protein seen in the nucleusof HeLa cells, and in the ER and cytoplasm of MCF7 cells. Recommended concentration : 10ug/mL.
• Immunogen: Peptide with sequence C-SETARSVPDMDLS, from the internal region of the protein sequence according to NP_005243.1.
• Preparation and Storage: Aliquot and store at -20 degrees C. Minimize freezing and thawing.

IHC (Immunohistchemistry)

(Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.)

IHC (Immunohistochemistry)

((8ug/ml) staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.)

IF (Immunofluorescence)

(Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)

IF (Immunofluorescence)

(Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and weak cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml))

IHC (Immunohistochemistry)

(AAA13673 (5ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 4885241
• NCBI GeneID: 2353
• NCBI Accession #: NP_005243.1
• NCBI GenBank Nucleotide #: NM_005252.3
• NCBI Official Full Name: proto-oncogene c-Fos
• NCBI Official Synonym Full Names: Fos proto-oncogene, AP-1 trancription factor subunit
• NCBI Official Symbol: FOS
• NCBI Official Synonym Symbols: p55; AP-1; C-FOS
• NCBI Protein Information: proto-oncogene c-Fos
• UniProt Protein Name: Proto-oncogene c-Fos
• UniProt Gene Name: FOS
• UniProt Synonym Gene Names: G0S7
• UniProt Entry Name: FOS_HUMAN