FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fo)
Goat Doublecortin/DCX Antibody | anti-DCX antibody
Goat Anti-Doublecortin/DCX (aa69-81) Antibody
Gene Names
DCX; DC; DBCN; LISX; SCLH; XLIS
Reactivity
Human, MouseExpected from sequence similarity: Human, Cow
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Doublecortin/DCX; N/A; Goat Anti-Doublecortin/DCX (aa69-81) Antibody; DCX; doublecortin; RP5-914P14.1; DBCN; DC; FLJ51296; LISX; SCLH; XLIS; OTTHUMP00000023860; OTTHUMP00000216316; doublecortex; doublin; lis-X; lissencephalin-X; neuronal migration protein doublecortin; anti-DCX antibody
Host
Goat
Reactivity
Human, Mouse
Expected from sequence similarity: Human, Cow
Expected from sequence similarity: Human, Cow
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence Length
441
Applicable Applications for anti-DCX antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ELISA (Peptide ELISA)
Immunogen
Peptide with sequence DRSHCQSLRFHQN, from the internal region of the protein sequence according to NP_000546.2.
Peptide Sequence
DRSHCQSLRFHQN
Epitope
Internal region
Important comments
This antibody is expected to recognize reported isoform a (NP_000546.2) only.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fo)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fo)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI ()
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI ()
References
Evangelisti C, Florian MC, Massimi I, Dominici C, Giannini G, Galardi S, Bu¨ MC, Massalini S, McDowell HP, Messi E, Gulino A, Farace MG, Ciafr SA.: MiR-128 up-regulation inhibits Reelin and DCX expression and reduces neuroblastoma cell motility and invasiveness. The FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2009 Dec 23 (12): 4276-87. PMID: 19713529
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
neuronal migration protein doublecortin isoform a
NCBI Official Synonym Full Names
doublecortin
NCBI Official Symbol
DCX
NCBI Official Synonym Symbols
DC; DBCN; LISX; SCLH; XLIS
NCBI Protein Information
neuronal migration protein doublecortin
UniProt Protein Name
Neuronal migration protein doublecortin
UniProt Gene Name
DCX
UniProt Synonym Gene Names
DBCN; LISX; Lis-X
UniProt Entry Name
DCX_HUMAN
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Product Notes
The DCX dcx (Catalog #AAA61838) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Anti-Doublecortin/DCX (aa69-81) Antibody reacts with Human, Mouse Expected from sequence similarity: Human, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's Doublecortin/DCX can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ELISA (Peptide ELISA). Researchers should empirically determine the suitability of the DCX dcx for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Doublecortin/DCX, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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