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product-image-AAA61820_IHC8.jpg IHC (Immunohistochemistry) ((3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

Goat EHD1 Antibody | anti-EHD1 antibody

Goat Anti-EHD1 Antibody

Gene Names
EHD1; PAST; PAST1; H-PAST; HPAST1
Reactivity
Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Cow
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
Synonyms
EHD1; N/A; Goat Anti-EHD1 Antibody; PAST; PAST1; H-PAST; HPAST1; EH-domain containing 1; testilin; FLJ42622; FLJ44618; OTTHUMP00000069747; anti-EHD1 antibody
Ordering
Host
Goat
Reactivity
Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Cow
Sequence Length
534
Applicable Applications for anti-EHD1 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IF (Immunofluorescence), WB (Western Blot), ELISA (Peptide ELISA)
Peptide Sequence
FSWVSKDARRKKEPC
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

IHC (Immunohistochemistry)

((3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

product-image-AAA61820_IHC8.jpg IHC (Immunohistochemistry) ((3.75ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

IHC (Immunohistochemistry)

((3.75ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

product-image-AAA61820_IHC10.jpg IHC (Immunohistochemistry) ((3.75ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)

product-image-AAA61820_FCM11.jpg FCM/FACS (Flow Cytometry) (Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)

IF (Immunofluorescence)

(Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)

product-image-AAA61820_IF13.jpg IF (Immunofluorescence) (Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)

WB (Western Blot)

((0.1ug/ml) staining of NIH3T3 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)

product-image-AAA61820_WB15.jpg WB (Western Blot) ((0.1ug/ml) staining of NIH3T3 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
EH domain-containing protein 1 isoform 1
NCBI Official Synonym Full Names
EH domain containing 1
NCBI Official Symbol
EHD1
NCBI Official Synonym Symbols
PAST; PAST1; H-PAST; HPAST1
NCBI Protein Information
EH domain-containing protein 1
UniProt Protein Name
EH domain-containing protein 1
UniProt Gene Name
EHD1
UniProt Synonym Gene Names
PAST; PAST1; hPAST1
UniProt Entry Name
EHD1_HUMAN

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Product Notes

The EHD1 ehd1 (Catalog #AAA61820) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Anti-EHD1 Antibody reacts with Human, Mouse Expected from sequence similarity: Human, Mouse, Rat, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's EHD1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IF (Immunofluorescence), WB (Western Blot), ELISA (Peptide ELISA). Researchers should empirically determine the suitability of the EHD1 ehd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EHD1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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