Goat GFAP Antibody | anti-GFAP antibody
Goat anti-GFAP Antibody
Gene Names
GFAP; ALXDRD
Reactivity
Tested: Human, RatExpected from sequence similarity: Human, Mouse, Rat, Dog
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
GFAP; N/A; Goat anti-GFAP Antibody; glial fibrillary acidic protein; FLJ45472; intermediate filament protein antibody; anti-GFAP antibody
Host
Goat
Reactivity
Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog
Expected from sequence similarity: Human, Mouse, Rat, Dog
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Applicable Applications for anti-GFAP antibody
Peptide ELISA, Western Blot (WB)
Application Notes
Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 48kDa band observed in Human Cerebellum and Mouse Brain lysates, and approx 50kDa band in Rat Brain lysates (calculated MW of 49.9kDa according to Human NP_002046.1, Mouse NP_034407.2 and Rat NP_058705.2). Recommended concentration: 0.005-0.1µg/ml. Primary incubation 1 hour at room temperature
Western blot: Approx 48kDa band observed in Human Cerebellum and Mouse Brain lysates, and approx 50kDa band in Rat Brain lysates (calculated MW of 49.9kDa according to Human NP_002046.1, Mouse NP_034407.2 and Rat NP_058705.2). Recommended concentration: 0.005-0.1µg/ml. Primary incubation 1 hour at room temperature
Immunogen
Peptide with sequence C-DGEVIKESKQEHKD, from the C Terminus of the protein sequence according to NP_002046.1.
Important Comments
GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells but its exact function remains poorly understood, despite the number of studies using it as a cell marker.
Preparation and Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
glial fibrillary acidic protein isoform 1
NCBI Official Synonym Full Names
glial fibrillary acidic protein
NCBI Official Symbol
GFAP
NCBI Official Synonym Symbols
ALXDRD
NCBI Protein Information
glial fibrillary acidic protein
UniProt Protein Name
Glial fibrillary acidic protein
UniProt Gene Name
GFAP
UniProt Synonym Gene Names
GFAP
UniProt Entry Name
GFAP_HUMAN
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Product Notes
The GFAP gfap (Catalog #AAA13651) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-GFAP Antibody reacts with Tested: Human, Rat Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's GFAP can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA, Western Blot (WB). Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 48kDa band observed in Human Cerebellum and Mouse Brain lysates, and approx 50kDa band in Rat Brain lysates (calculated MW of 49.9kDa according to Human NP_002046.1, Mouse NP_034407.2 and Rat NP_058705.2). Recommended concentration: 0.005-0.1µg/ml. Primary incubation 1 hour at room temperature. Researchers should empirically determine the suitability of the GFAP gfap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GFAP, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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