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IHC (Immunohistchemistry)
((3.75ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Goat KCNN4/KCa3.1 Antibody | anti-KCNN4 antibody
Goat Anti-KCNN4/KCa3.1 Antibody
Gene Names
KCNN4; IK; IK1; SK4; DHS2; KCA4; hSK4; IKCA1; hKCa4; KCa3.1; hIKCa1
Reactivity
Human, MouseExpected from sequence similarity: Human, Mouse, Rat, Dog
Applications
ELISA, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
KCNN4/KCa3.1; N/A; Goat Anti-KCNN4/KCa3.1 Antibody; KCNN4; potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4; IK1; IKCA1; KCA4; KCa3.1; SK4; hIKCa1; hKCa4; hSK4; intermediate conductance calcium-activated potassium channel protein 1; putative erythrocyte intermediate conductance calcium-activated potassium Gardos channel; anti-KCNN4 antibody
Host
Goat
Reactivity
Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog
Expected from sequence similarity: Human, Mouse, Rat, Dog
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence Length
427
Applicable Applications for anti-KCNN4 antibody
Peptide ELISA (EIA), WB, Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
Application Notes
Peptide ELISA: antibody detection limit dilution 1:16000
WB: Approx 50kDa band observed in Human Cerebellum lysates and in lysates of cell line NIH3T3 (calculated MW of 47.7kDa, according to Human NP_002241.1 and Mouse NP_032459.3).An additional band of unknown identity was also consistently observed at 35kDa in Cerebellum. This band was successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Adrenal Gland lysate.
IHC: Paraffin embedded Human Adrenal Gland, Kidney and Liver. Recommended concentration: 3.75ug/ml.
IF: Strong expression of the protein seen in the cytoplasm/membranes of HeLa cells. Recommended concentration: 10ug/ml.
FC/FACS: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.
WB: Approx 50kDa band observed in Human Cerebellum lysates and in lysates of cell line NIH3T3 (calculated MW of 47.7kDa, according to Human NP_002241.1 and Mouse NP_032459.3).An additional band of unknown identity was also consistently observed at 35kDa in Cerebellum. This band was successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Adrenal Gland lysate.
IHC: Paraffin embedded Human Adrenal Gland, Kidney and Liver. Recommended concentration: 3.75ug/ml.
IF: Strong expression of the protein seen in the cytoplasm/membranes of HeLa cells. Recommended concentration: 10ug/ml.
FC/FACS: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.
Immunogen
Peptide with sequence C-ERQAVNATGHLSD, from the internal region of the protein sequence according to NP_002241.1.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
IHC (Immunohistchemistry)
((3.75ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
IHC (Immunohistchemistry)
((3.75ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
IHC (Immunohistochemistry)
((3.75ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
IHC (Immunohistochemistry)
((3.75ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
IHC (Immunohistochemistry)
((3.75ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
IHC (Immunohistochemistry)
((3.75ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
FCM (Flow Cytometry)
(Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight at 4 degree C (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG ()
FCM (Flow Cytometry)
(Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight at 4 degree C (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG ()
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane/cytoplasmic staining. The nuclear stain i)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane/cytoplasmic staining. The nuclear stain i)
WB (Western Blot)
((0.1ug/ml) staining of Human Cerebellum (A) + Peptide (B), NIH3T3 (C) and negative Control Adrenal Gland (D) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
WB (Western Blot)
((0.1ug/ml) staining of Human Cerebellum (A) + Peptide (B), NIH3T3 (C) and negative Control Adrenal Gland (D) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
References
Yamaguchi M, Nakayama T, Fu Z, Naganuma T, Sato N, Soma M, Doba N, Hinohara S, Morita A, Mizutani T.: Relationship between haplotypes of KCNN4 gene and susceptibility to human vascular diseases in Japanese. Medical science monitor: international medical journal of experimental and clinical research 2009 Aug 15 (8): CR389-97. PMID: 19644414
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
intermediate conductance calcium-activated potassium channel protein 4
NCBI Official Synonym Full Names
potassium calcium-activated channel subfamily N member 4
NCBI Official Symbol
KCNN4
NCBI Official Synonym Symbols
IK; IK1; SK4; DHS2; KCA4; hSK4; IKCA1; hKCa4; KCa3.1; hIKCa1
NCBI Protein Information
intermediate conductance calcium-activated potassium channel protein 4
UniProt Protein Name
Intermediate conductance calcium-activated potassium channel protein 4
UniProt Gene Name
KCNN4
UniProt Synonym Gene Names
IK1; IKCA1; KCA4; SK4; SK4; SKCa 4; SKCa4; IK1
UniProt Entry Name
KCNN4_HUMAN
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Product Notes
The KCNN4 kcnn4 (Catalog #AAA13678) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Anti-KCNN4/KCa3.1 Antibody reacts with Human, Mouse Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's KCNN4/KCa3.1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), WB, Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC). Peptide ELISA: antibody detection limit dilution 1:16000 WB: Approx 50kDa band observed in Human Cerebellum lysates and in lysates of cell line NIH3T3 (calculated MW of 47.7kDa, according to Human NP_002241.1 and Mouse NP_032459.3).An additional band of unknown identity was also consistently observed at 35kDa in Cerebellum. This band was successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Adrenal Gland lysate. IHC: Paraffin embedded Human Adrenal Gland, Kidney and Liver. Recommended concentration: 3.75ug/ml. IF: Strong expression of the protein seen in the cytoplasm/membranes of HeLa cells. Recommended concentration: 10ug/ml. FC/FACS: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml. Researchers should empirically determine the suitability of the KCNN4 kcnn4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KCNN4/KCa3.1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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