Goat Beta-casein (CSN2) ELISA Kit | CSN2 elisa kit
Goat Beta-casein (CSN2) ELISA Kit
Gene Names
CSN2; CASB
Reactivity
Goat
Synonyms
Beta-casein (CSN2); N/A; Goat Beta-casein (CSN2) ELISA Kit; CSN2 elisa kit
Reactivity
Goat
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
1.0 ug/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for CSN2 elisa kit
Intended Uses: This CSN2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat CSN2. This ELISA kit for research use only!
Principle of the Assay||CSN2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CSN2 antibody and an CSN2-HRP conjugate. The assay sample and buffer are incubated together with CSN2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CSN2 concentration since CSN2 from samples and CSN2-HRP conjugate compete for the anti-CSN2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CSN2 from the sample, fewer sites are left to bind CSN2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CSN2 concentration in each sample is interpolated from this standard curve.
Principle of the Assay||CSN2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CSN2 antibody and an CSN2-HRP conjugate. The assay sample and buffer are incubated together with CSN2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CSN2 concentration since CSN2 from samples and CSN2-HRP conjugate compete for the anti-CSN2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CSN2 from the sample, fewer sites are left to bind CSN2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CSN2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,382 Da
NCBI Official Full Name
beta-casein
NCBI Official Synonym Full Names
casein beta
NCBI Official Symbol
CSN2
NCBI Official Synonym Symbols
CASB
NCBI Protein Information
beta-casein
UniProt Protein Name
Beta-casein
UniProt Gene Name
CSN2
UniProt Synonym Gene Names
CASB
UniProt Entry Name
CASB_HUMAN
