FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fo)
Goat anti-Human GLI1 Antibody | anti-GLI1 antibody
Goat Anti-GLI1 Antibody
Gene Names
GLI1; GLI; PPD1; PAPA8
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
GLI1; N/A; Goat Anti-GLI1 Antibody; GLI family zinc finger 1; GLI; glioma-associated oncogene family zinc finger 1; glioma-associated oncogene homolog 1 (zinc finger protein); oncogene GLI; zinc finger protein GLI1; anti-GLI1 antibody
Host
Goat
Reactivity
Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence Length
1106
Applicable Applications for anti-GLI1 antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ELISA (Peptide ELISA)
Immunogen
Peptide with sequence TYSQCPRLEHYGQ, from the internal region of the protein sequence according to NP_005260.1; NP_001153517.1; NP_001161081.1.
Peptide Sequence
TYSQCPRLEHYGQ
Epitope
Internal region
Important comments
This antibody is expected to recognize the reported isoforms (NP_005260.1; NP_001153517.1; NP_001161081.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fo)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fo)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (b)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
zinc finger protein GLI1 isoform 1
NCBI Official Synonym Full Names
GLI family zinc finger 1
NCBI Official Symbol
GLI1
NCBI Official Synonym Symbols
GLI; PPD1; PAPA8
NCBI Protein Information
zinc finger protein GLI1
UniProt Protein Name
Zinc finger protein GLI1
UniProt Gene Name
GLI1
UniProt Synonym Gene Names
GLI
UniProt Entry Name
GLI1_HUMAN
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Product Notes
The GLI1 gli1 (Catalog #AAA61837) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Anti-GLI1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GLI1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ELISA (Peptide ELISA). Researchers should empirically determine the suitability of the GLI1 gli1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GLI1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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