IF (Immunofluorescence)
(EB11821 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmicstaining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
Goat PROM1/CD133 Antibody | anti-PROM1 antibody
Goat Anti-PROM1/CD133 Antibody
Gene Names
PROM1; RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061
Reactivity
HumanExpected from sequence similarity: Human
Applications
Immunofluorescence, ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
PROM1/CD133; N/A; Goat Anti-PROM1/CD133 Antibody; AC133, antigen AC133, CD133, CORD12, hematopoietic stem cell antigen, hProminin, MCDR2, OTTHUMP00000217744, OTTHUMP00000217745, OTTHUMP00000217746, PROM1, prominin 1, prominin-1, prominin-like 1, prominin-like protein 1, PROML1, RP41, STGD4; anti-PROM1 antibody
Host
Goat
Reactivity
Human
Expected from sequence similarity: Human
Expected from sequence similarity: Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence Length
865
Applicable Applications for anti-PROM1 antibody
IF (Immunofluorescence), ELISA (Peptide ELISA)
Immunogen
Peptide with sequence RHATPTTRGCVSN, from the internal region of the protein sequence according to NP_006008.1; NP_001139319.1; NP_001139324.1; NP_001139323.1; NP_001139322.1; NP_001139321.1.
Peptide Sequence
RHATPTTRGCVSN
Important comments
This antibody is expected to recognize all reported isoforms (NP_006008.1; NP_001139319.1; NP_001139324.1; NP_001139323.1; NP_001139322.1; NP_001139321.1). Reported variants represent identical protein: NP_001139320.1, NP_001139319.1
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
IF (Immunofluorescence)
(EB11821 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmicstaining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
IF (Immunofluorescence)
(EB11821 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmicstaining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
References
Bourseau-Guilmain E, Griveau A, Benoit JP, Garcion E.: The importance of the stem cell marker prominin-1/CD133 in the uptake of transferrin and in iron metabolism in human colon cancer Caco-2 cells. PLoS One. 2011;6(9):e25515. PMID: 21966538
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
prominin-1 isoform 1
NCBI Official Synonym Full Names
prominin 1
NCBI Official Symbol
PROM1
NCBI Official Synonym Symbols
RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061
NCBI Protein Information
prominin-1
UniProt Protein Name
Prominin-1
UniProt Gene Name
PROM1
UniProt Synonym Gene Names
PROML1
UniProt Entry Name
PROM1_HUMAN
Similar Products
Product Notes
The PROM1 prom1 (Catalog #AAA61842) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Anti-PROM1/CD133 Antibody reacts with Human Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's PROM1/CD133 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA (Peptide ELISA). Researchers should empirically determine the suitability of the PROM1 prom1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PROM1/CD133, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.