Goat TNFSF11/OPGL Antibody | anti-TNFSF11 antibody

Goat Anti-TNFSF11/OPGL Antibody

Cat. Num. AAA61824

• Gene Names: TNFSF11; ODF; OPGL; sOdf; CD254; OPTB2; RANKL; TNLG6B; TRANCE; hRANKL2
• Reactivity: Human, Mouse; Expected from sequence similarity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: TNFSF11/OPGL; N/A; Goat Anti-TNFSF11/OPGL Antibody; TNFSF11; tumor necrosis factor (ligand) superfamily, member 11 ; ODF; OPGL; RANKL; TRANCE; hRANKL2; sOdf; TNF-related activation-induced cytokine; osteoclast differentiation factor; osteoprotegerin ligand; receptor activator of nuclear factor kappa B ligand tumor necrosis factor ligand superfamily, member 11; anti-TNFSF11 antibody

• Host: Goat
• Reactivity: Human, Mouse; Expected from sequence similarity: Human
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Sequence Length: 317

• Applicable Applications for anti-TNFSF11 antibody: FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ELISA (Peptide ELISA)
• Immunogen: Peptide with sequence C-DLAKRSKLEAQP, from the internal region of the protein sequence according to NP_003692.1; NP_143026.1.
• Peptide Sequence: DLAKRSKLEAQP
• Epitope: Internal region
• Important comments: This antibody is expected to recognise both reported isoforms (NP_003692.1 and NP_143026.1).
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black lin)

IF (Immunofluorescence)

(Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and secreted staining. The nuclear sta)

IF (Immunofluorescence)

(Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and secreted staining. The nuclear stain)

References

Kim K, Kim JH, Lee J, Jin HM, Lee SH, Fisher DE, Kook H, Kim KK, Choi Y, Kim N.: Nuclear factor of activated T cells c1 induces osteoclast-associated receptor gene expression during tumor necrosis factor-related activation-induced cytokine-mediated osteoclastogenesis. J Biol Chem. 2005 Oct 21;280(42):35209-16. Epub 2005 Aug 18. PMID: 16109714

NCBI and Uniprot Product Information

• NCBI GI #: 4507595
• NCBI GeneID: 8600
• NCBI Accession #: NP_003692.1; NP_143026.1
• NCBI GenBank Nucleotide #: NP_003692.1
• UniProt Accession #: O14788
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 11 isoform 1
• NCBI Official Synonym Full Names: TNF superfamily member 11
• NCBI Official Symbol: TNFSF11
• NCBI Official Synonym Symbols: ODF; OPGL; sOdf; CD254; OPTB2; RANKL; TNLG6B; TRANCE; hRANKL2
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 11
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 11
• UniProt Gene Name: TNFSF11
• UniProt Synonym Gene Names: OPGL; RANKL; TRANCE; ODF; OPGL; RANKL; TRANCE
• UniProt Entry Name: TNF11_HUMAN