IHC (Immunohistochemistry)
(Immunohistochemistry analysis of HRP Goat Anti-Rabbit IgG (H+L) in paraffin-embedded human testis tissue using HRP Goat Anti-Rabbit IgG (H+L) (AAA10763) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)
IgG (H+L) Secondary Antibody
HRP Goat Anti-Rabbit IgG (H+L) Antibody
Reactivity
Rabbit
Applications
ELISA, Western Blot, Immunohistochemistry, Dot Blot
Purity
Affinity Purification
Synonyms
IgG (H+L); N/A; HRP Goat Anti-Rabbit IgG (H+L) Antibody; HRP Goat Anti-Rabbit IgG (H+L): N/A; IgG (H+L) secondary antibody
Host
Goat
Reactivity
Rabbit
Isotype
Horseradish peroxidase conjugated IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.75% BSA, 50% glycerol, pH 7.3.
Applicable Applications for IgG (H+L) secondary antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry Parrafin (IHC-P), Dot Blot (DB)
Application Notes
ELISA: 1:5000 - 1:10000
WB: 1:2000 - 1:10000
IHC: 1:50 - 1:200
IHC-P: 1:50 - 1:200
WB: 1:2000 - 1:10000
IHC: 1:50 - 1:200
IHC-P: 1:50 - 1:200
Immunogen
Rabbit IgG
Conjugation
HRP
Preparation and Storage
Store at -20°C. Avoid freeze/thaw cycles.
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of HRP Goat Anti-Rabbit IgG (H+L) in paraffin-embedded human testis tissue using HRP Goat Anti-Rabbit IgG (H+L) (AAA10763) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of HRP Goat Anti-Rabbit IgG (H+L) in paraffin-embedded human testis tissue using HRP Goat Anti-Rabbit IgG (H+L) (AAA10763) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)
WB (Western Blot)
(Western blot analysis of extracts of HeLa cells,using GAPDH antibody as the primary antibody at dilution of 1:80000.Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody ( AAA10763 ) at 1:4000-1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s)
WB (Western Blot)
(Western blot analysis of extracts of HeLa cells,using GAPDH antibody as the primary antibody at dilution of 1:80000.Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody ( AAA10763 ) at 1:4000-1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s)
Related Product Information for IgG (H+L) secondary antibody
Background: Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
Product Categories/Family for IgG (H+L) secondary antibody
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Product Notes
The IgG (H+L) (Catalog #AAA10763) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The HRP Goat Anti-Rabbit IgG (H+L) Antibody reacts with Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's IgG (H+L) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry Parrafin (IHC-P), Dot Blot (DB). ELISA: 1:5000 - 1:10000 WB: 1:2000 - 1:10000 IHC: 1:50 - 1:200 IHC-P: 1:50 - 1:200. Researchers should empirically determine the suitability of the IgG (H+L) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG (H+L), Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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