Standard Curve (Sample)
Chicken Glucoprotein 130 ELISA Kit | gp130 elisa kit
Chicken Glucoprotein 130 ELISA Kit
Gene Names
IL6ST; CD130; GP130; CDW130; IL-6RB
Reactivity
Chicken
Synonyms
Glucoprotein 130; N/A; Chicken Glucoprotein 130 ELISA Kit; gp130 elisa kit
Reactivity
Chicken
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
1.0 ng/mL.
Intended Uses
This gp130 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken gp130. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for gp130 elisa kit
Principle of the assay: gp130 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-gp130 antibody and an gp130-HRP conjugate. The assay sample and buffer are incubated together with gp130-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the gp130 concentration since gp130 from samples and gp130-HRP conjugate compete for the anti-gp130 antibody binding site. Since the number of sites is limited, as more sites are occupied by gp130 from the sample, fewer sites are left to bind gp130-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The gp130 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for gp130 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
5,511 Da
NCBI Official Full Name
gp130
NCBI Official Synonym Full Names
interleukin 6 signal transducer (gp130, oncostatin M receptor)
NCBI Official Symbol
IL6ST
NCBI Official Synonym Symbols
CD130; GP130; CDW130; IL-6RB
NCBI Protein Information
interleukin-6 receptor subunit beta; CD130 antigen; IL-6R subunit beta; membrane glycoprotein 130; IL-6 receptor subunit beta; membrane glycoprotein gp130; interleukin receptor beta chain; oncostatin-M receptor subunit alpha; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form
UniProt Protein Name
Gp130
UniProt Gene Name
gp130
UniProt Entry Name
Q13514_HUMAN
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Product Notes
The Chicken gp130 gp130 (Catalog #AAA88179) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA88179 ELISA Kit recognizes Chicken gp130. It is sometimes possible for the material contained within the vial of "Glucoprotein 130, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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