Human G protein-coupled bile acid receptor 1 ELISA Kit | GPBAR1 elisa kit

Human G protein-coupled bile acid receptor 1, GPBAR1 ELISA Kit

Cat. Num. AAA79568

• Gene Names: GPBAR1; BG37; TGR5; M-BAR; GPCR19; GPR131
• Reactivity: Human
• Synonyms: G protein-coupled bile acid receptor 1; N/A; Human G protein-coupled bile acid receptor 1, GPBAR1 ELISA Kit; Human G protein-coupled bile acid receptor 1 (GPBAR1) ELISA Kit; BG37; GPCR; GPCR19; GPR131; M-BAR; MGC40597; TGR5; G-protein coupled bile acid receptor BG37; membrane bile acid receptor; membrane-type receptor for bile acids; GPBAR1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human GPBAR1. No significant cross-reactivity or interference between Human GPBAR1 and analogues was observed.
• Sequence Length: 330
• Detection Range: 0.156 ng/ml-10 ng/ml
• Sensitivity: 0.039 ng/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for GPBAR1 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GPBAR1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GPBAR1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain GPBAR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of GPBAR1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 116284384
• NCBI GeneID: 151306
• NCBI Accession #: NP_001070659.1
• NCBI GenBank Nucleotide #: NM_001077191.1
• UniProt Accession #: Q8TDU6; B3KV35
• Molecular Weight: 35,248 Da
• NCBI Official Full Name: G-protein coupled bile acid receptor 1
• NCBI Official Synonym Full Names: G protein-coupled bile acid receptor 1
• NCBI Official Symbol: GPBAR1
• NCBI Official Synonym Symbols: BG37; TGR5; M-BAR; GPCR19; GPR131
• NCBI Protein Information: G-protein coupled bile acid receptor 1; hBG37; hGPCR19; membrane bile acid receptor; G-protein coupled receptor GPCR19; membrane-type receptor for bile acids; G-protein coupled bile acid receptor BG37
• UniProt Protein Name: G-protein coupled bile acid receptor 1
• UniProt Gene Name: GPBAR1
• UniProt Synonym Gene Names: TGR5; hGPCR19; M-BAR; BG37
• UniProt Entry Name: GPBAR_HUMAN