Human Glycogen Phosphorylase lsoenzyme BB ELISA Kit | GPBB elisa kit

Human Glycogen Phosphorylase lsoenzyme BB ELISA Kit

Cat. Num. AAA16538

• Gene Names: PYGB; GPBB
• Reactivity: Human
• Synonyms: Glycogen Phosphorylase lsoenzyme BB; N/A; Human Glycogen Phosphorylase lsoenzyme BB ELISA Kit; GPBB elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of GPBB. No significant cross-reactivity or interference between GPBB and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GPBB and all the analogues, therefore, cross reaction may still exist in some cases.
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive or Sandwich
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for GPBB elisa kit

Intended Uses: This GPBB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GPBB. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: GPBB ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GPBB. Standards or samples are then added to the microtiter plate wells and GPBB if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GPBB present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GPBB are added to each well to "sandwich" the GPBB immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GPBB and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GPBB concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 21361370
• NCBI GeneID: 5834
• NCBI Accession #: NP_002853.2
• NCBI GenBank Nucleotide #: NM_002862.3
• Molecular Weight: 96,696 Da
• NCBI Official Full Name: glycogen phosphorylase, brain form
• NCBI Official Synonym Full Names: phosphorylase, glycogen; brain
• NCBI Official Symbol: PYGB
• NCBI Official Synonym Symbols: GPBB
• NCBI Protein Information: glycogen phosphorylase, brain form; glycogen phosphorylase B
• UniProt Protein Name: Glycogen phosphorylase, brain form
• UniProt Gene Name: PYGB
• UniProt Entry Name: PYGB_HUMAN