Granulin (GRN) Recombinant Protein | GRN recombinant protein
Recombinant Granulin (GRN)
Gene Names
GRN; GEP; GP88; PEPI; PGRN; CLN11; PCDGF
Reactivity
Homo sapiens (Human)
Applications
SDS-Page, Western Blot
Purity
> 95%
Synonyms
Granulin (GRN); N/A; Recombinant Granulin (GRN); GRN recombinant protein
Host
E.coli
Reactivity
Homo sapiens (Human)
Purity/Purification
> 95%
Form/Format
PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Concentration
Original Concentration: 120ug/mL (varies by lot)
Applicable Applications for GRN recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
Application Notes
(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression
Residues
Met234~Gly419
Tags
N-terminal His Tag
Subcellular Location
Secreted
Traits
Freeze-dried powder
Predicted isoelectric point
5.2
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
SDS-PAGE
Gene Sequencing (Extract)
Sequence Information
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted Molecular Mass: 26.7kDa
Accurate Molecular Mass: 34kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 34kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
granulins
NCBI Official Synonym Full Names
granulin
NCBI Official Symbol
GRN
NCBI Official Synonym Symbols
GEP; GP88; PEPI; PGRN; CLN11; PCDGF
NCBI Protein Information
granulins; acrogranin; progranulin; proepithelin; granulin-epithelin; PC cell-derived growth factor
UniProt Protein Name
Granulins
UniProt Gene Name
GRN
UniProt Synonym Gene Names
PEPI
UniProt Entry Name
GRN_HUMAN
