Standard Curve (Sample)
Human Gelsolin ELISA Kit | GSN elisa kit
Human Gelsolin ELISA Kit
Gene Names
GSN; ADF; AGEL
Reactivity
Human
Synonyms
Gelsolin; N/A; Human Gelsolin ELISA Kit; AGEL; Actin-depolymerizing factor; ADF; Brevin; GSN; GSN elisa kit
Reactivity
Human
Specificity
Recombinant and natural Human Gelsolin
Sequence Length
782
Assay Type
Sandwich
Detection Range
0.78-50 ng/mL
Sensitivity
0.22ng/mL
Intra-Assay CV
<=5.1%
Inter-Assay CV
<=7.7%
Recovery
81%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for GSN elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates,cell culture supernates or other biological fluids.
Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen, During the reaction, target antigen in the sample or standard competes with a fixed amount of biotin-labeled target antigen for sites on a pre-coated antibody specific to target antigen. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen, During the reaction, target antigen in the sample or standard competes with a fixed amount of biotin-labeled target antigen for sites on a pre-coated antibody specific to target antigen. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
81,485 Da
NCBI Official Full Name
gelsolin isoform a
NCBI Official Synonym Full Names
gelsolin
NCBI Official Symbol
GSN
NCBI Official Synonym Symbols
ADF; AGEL
NCBI Protein Information
gelsolin
UniProt Protein Name
Gelsolin
UniProt Gene Name
GSN
UniProt Synonym Gene Names
ADF
UniProt Entry Name
GELS_HUMAN
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Product Notes
The Human GSN gsn (Catalog #AAA194278) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA194278 ELISA Kit recognizes Human GSN. It is sometimes possible for the material contained within the vial of "Gelsolin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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