Mouse Glycosylated serum protein ELISA Kit | GSP elisa kit

Mouse Glycosylated serum protein ELISA Kit

Cat. Num. AAA86756

• Reactivity: Mouse
• Synonyms: Glycosylated serum protein; N/A; Mouse Glycosylated serum protein ELISA Kit; GSP elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of GSP. No significant cross-reactivity or interference between GSP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GSP and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 mmol/L

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for GSP elisa kit

Principle of the Assay: GSP ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-GSP antibody and an GSP-HRP conjugate. The assay sample and buffer are incubated together with GSP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GSP concentration since GSP from samples and GSP-HRP conjugate compete for the anti-GSP antibody binding site. Since the number of sites is limited, as more sites are occupied by GSP from the sample, fewer sites are left to bind GSP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GSP concentration in each sample is interpolated from this standard curve.

Intended Uses: This GSP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse GSP. This ELISA kit for research use only, not for therapeutic or test applications!

Product Categories/Family for GSP elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 474185
• UniProt Accession #: P40683
• Molecular Weight: 27,857 Da
• NCBI Official Full Name: GSP
• UniProt Protein Name: 4'-phosphopantetheinyl transferase gsp
• UniProt Gene Name: gsp
• UniProt Entry Name: GSP_ANEMI