ELISA
( Well Number Concentration A450 1 62.5 ng/mL >3.8 2 31.3 ng/mL 3.150 3 15.7 ng/mL 2.084 4 7.9 ng/mL 1.054 5 4.0 ng/mL 0.638 6 2.0 ng/mL 0.282 7 1.0 ng/mL 0.153 8 0.5 ng/mL 0.091 9 0 ng/mL 0.036 QC Release Date: 06-27-2024 )
Glutathione S-Transferase P (GSTp) Recombinant Protein | GSTp recombinant protein
Purified Glutathione S-Transferase P (GSTp) CHO-S
Gene Names
GSTP1; PI; DFN7; GST3; GSTP; FAEES3; HEL-S-22
Reactivity
CHO, Hamster
Applications
Western Blot, ELISA
Synonyms
Glutathione S-Transferase P (GSTp); N/A; Purified Glutathione S-Transferase P (GSTp) CHO-S; Purified CHO GSTp Protein; GSTp recombinant protein
Host
CHO-S Cells
Reactivity
CHO, Hamster
Specificity
The protein is detected by Western blotting using anti-6His and anti-GSTP antibodies. The product concentration is determined using absorbance reading at 280 nm (A0.1%280 value = 1.1) and a Bradford protein assay with a BSA standard
curve.
Form/Format
Liquid; PBS buffer, pH 7.2, with non-protein stabilizer
Concentration
0.56 mg/ml (varies by lot)
Sequence Length
210
Applicable Applications for GSTp recombinant protein
WB (Western Blot), ELISA
Physical State
Frozen Liquid
Preservative
0.09% sodium azide
Procedure
Recombinant GSTP (GenBank: AAB39859.1) with a 6xHIS tag was expressed in and purified from CHO-S cells. Under reducing conditions, GSTP is detected at an estimated molecular weight of 25 kDa
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Quality Control Testing
Quality control tested by Direct ELISA utilizing the following parameters.
| Step | Action | Materials Used |
|---|---|---|
| 1 | Antigen @ 25 ng/well. Incubated 60 minutes. | Purified CHO GSTp Protein |
| 2 | Block plates for 30 minutes | 1% BSA |
| 3 | HRP-Conjugated Antibody. Incubated 60 minutes | HRP Rabbit anti-6xHIS (RHIS-45P) |
| 4 | Develop with TMB. Incubated 10 minutes | TMB Substrate |
| 5 | Stop Reaction | 0.3M H2SO4 |
| 6 | Read plates at A450 | Microplate Reader set at 450nm |
Preparation and Storage
Store at -80°C. Following the initial thaw, please prepare multiple aliquots and store at -80°C. Multiple freeze-thaw cycles are not recommended.
ELISA
( Well Number Concentration A450 1 62.5 ng/mL >3.8 2 31.3 ng/mL 3.150 3 15.7 ng/mL 2.084 4 7.9 ng/mL 1.054 5 4.0 ng/mL 0.638 6 2.0 ng/mL 0.282 7 1.0 ng/mL 0.153 8 0.5 ng/mL 0.091 9 0 ng/mL 0.036 QC Release Date: 06-27-2024 )
ELISA
( Well Number Concentration A450 1 62.5 ng/mL >3.8 2 31.3 ng/mL 3.150 3 15.7 ng/mL 2.084 4 7.9 ng/mL 1.054 5 4.0 ng/mL 0.638 6 2.0 ng/mL 0.282 7 1.0 ng/mL 0.153 8 0.5 ng/mL 0.091 9 0 ng/mL 0.036 QC Release Date: 06-27-2024 )
Related Product Information for GSTp recombinant protein
Purified recombinant Chinese hamster ovary Glutathione S-Transferase Pi (GSTp) Protein expressed in CHO-S cells. Contains a 6xHIS tag.
Product Categories/Family for GSTp recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
glutathione S-transferase P
NCBI Official Synonym Full Names
glutathione S-transferase pi 1
NCBI Official Symbol
GSTP1
NCBI Official Synonym Symbols
PI; DFN7; GST3; GSTP; FAEES3; HEL-S-22
NCBI Protein Information
glutathione S-transferase P
UniProt Protein Name
Glutathione S-transferase P
UniProt Gene Name
GSTP1
UniProt Synonym Gene Names
FAEES3; GST3
UniProt Entry Name
GSTP1_HUMAN
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Product Notes
The GSTp gstp1 (Catalog #AAA76817) is a Recombinant Protein produced from CHO-S Cells and is intended for research purposes only. The product is available for immediate purchase. The Purified Glutathione S-Transferase P (GSTp) CHO-S reacts with CHO, Hamster and may cross-react with other species as described in the data sheet. AAA Biotech's Glutathione S-Transferase P (GSTp) can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the GSTp gstp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glutathione S-Transferase P (GSTp), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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