Porcine Granzymes A ELISA Kit | Gzms-A elisa kit

Porcine Granzymes A ELISA Kit

Cat. Num. AAA92317

• Gene Names: GZMA; HFSP; CTLA3
• Reactivity: Porcine
• Synonyms: Granzymes A; N/A; Porcine Granzymes A ELISA Kit; Gzms-A elisa kit

• Reactivity: Porcine
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.

• Intended Uses: This GZMS-A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine GZMS-A. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for Gzms-A elisa kit

Principle of the Assay: GZMS-A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GZMS-A antibody and an GZMS-A-HRP conjugate. The assay sample and buffer are incubated together with GZMS-A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GZMS-A concentration since GZMS-A from samples and GZMS-A-HRP conjugate compete for the anti-GZMS-A antibody binding site. Since the number of sites is limited, as more sites are occupied by GZMS-A from the sample, fewer sites are left to bind GZMS-A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GZMS-A concentration in each sample is interpolated from this standard curve.

Product Categories/Family for Gzms-A elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 5453676
• NCBI GeneID: 3001
• NCBI Accession #: NP_006135.1
• NCBI GenBank Nucleotide #: NM_006144.3
• Molecular Weight: 27,109 Da
• NCBI Official Full Name: granzyme A
• NCBI Official Synonym Full Names: granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3)
• NCBI Official Symbol: GZMA
• NCBI Official Synonym Symbols: HFSP; CTLA3
• NCBI Protein Information: granzyme A; HF; h factor; CTL tryptase; fragmentin-1; cytotoxic T-lymphocyte proteinase 1; Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factor serine protease)
• UniProt Protein Name: Granzyme A
• UniProt Gene Name: GZMA
• UniProt Synonym Gene Names: CTLA3; HFSP; H factor; HF
• UniProt Entry Name: GRAA_HUMAN