Application Data
(Recombinant Mononucleosomes (H2A.Z) - biotinylated, DNA gel. Biotinylated mononucleosomes (H2A.Z) were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: free 601 DNA. Lane 3: Intact mononucleosomes. Intact mononucleosomes migrate much higher than free 601 DNA. The agarose gel result shows that almost all of 601 DNA wraps histone octamers to form mononucleosomes. Nucleosomes = Mononucleosomes.)
Mononucleosomes Recombinant Protein | H2AFZ recombinant protein
Recombinant Mononucleosomes (H2A.Z)-biotinylated
Gene Names
H2AFZ; H2AZ; H2A.z; H2A/z; H2A.Z-1
Synonyms
Mononucleosomes; N/A; Recombinant Mononucleosomes (H2A.Z)-biotinylated; H2A Histone Family Member Z; H2A/Z; H2AZ; H2AFZ recombinant protein
Host
E Coli
Form/Format
Recombinant Mononucleosomes (H2A.Z)-biotinylated (20ug protein + 20ug DNA) are supplied in 10mM Tris-HCl, pH8.0, 1mM EDTA, 2mM DTT and 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.
Protein Species
Human
Conjugation
Biotinylated
Protein Details
Recombinant Mononucleosomes (H2A.Z)-biotinylated, consist of 167 bp of 601 DNA with a 5' biotin tag and two molecules each of histones H2A.Z that includes amino acids 1-128 (end)(accession number NP_002097.1), H2B that includes amino acids 1-126 (end)(accession number NP_003509.1), H3 that includes amino acids 1-136 (end)(accession number NP_003520.1), and H4 that includes amino acids 1-103 (end)(accession number NP_003539.1). All of these histones were expressed in E Coli cells.
Notes
Recombinant Mononucleosomes (H2A.Z)-biotinylated are suitable for use as substrates in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(Recombinant Mononucleosomes (H2A.Z) - biotinylated, DNA gel. Biotinylated mononucleosomes (H2A.Z) were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: free 601 DNA. Lane 3: Intact mononucleosomes. Intact mononucleosomes migrate much higher than free 601 DNA. The agarose gel result shows that almost all of 601 DNA wraps histone octamers to form mononucleosomes. Nucleosomes = Mononucleosomes.)
Application Data
(Recombinant Mononucleosomes (H2A.Z) - biotinylated, DNA gel. Biotinylated mononucleosomes (H2A.Z) were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: free 601 DNA. Lane 3: Intact mononucleosomes. Intact mononucleosomes migrate much higher than free 601 DNA. The agarose gel result shows that almost all of 601 DNA wraps histone octamers to form mononucleosomes. Nucleosomes = Mononucleosomes.)
SDS-PAGE
(Streptavidin pull-down for Recombinant Mononucleosomes (H2A.Z) - biotinylated. Mononucleosomes (H2A.Z) were pulled down by streptavidin beads. Input mononucleosomes (Lane 1) and the mononucleosomes pulled down by streptavidin (Lane 2) were run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. The SDS-PAGE gel result shows that almost all of biotinylated mononucleosomes (H2A.Z) are pulled down by streptavidin beads. * indicates streptavidin. Nucleosomes = Mononucleosomes.)
SDS-PAGE
(Streptavidin pull-down for Recombinant Mononucleosomes (H2A.Z) - biotinylated. Mononucleosomes (H2A.Z) were pulled down by streptavidin beads. Input mononucleosomes (Lane 1) and the mononucleosomes pulled down by streptavidin (Lane 2) were run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. The SDS-PAGE gel result shows that almost all of biotinylated mononucleosomes (H2A.Z) are pulled down by streptavidin beads. * indicates streptavidin. Nucleosomes = Mononucleosomes.)
SDS-PAGE
(Recombinant Mononucleosomes (H2A.Z) - biotinylated, protein gel. 12.5% SDS-PAGE gel stained with Coomassie Blue Purity: ? 90%)
SDS-PAGE
(Recombinant Mononucleosomes (H2A.Z) - biotinylated, protein gel. 12.5% SDS-PAGE gel stained with Coomassie Blue Purity: ? 90%)
Related Product Information for H2AFZ recombinant protein
Short Description: Nucleosomes comprise the smallest subunit of chromatin; they consist of 147 bp of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). Therefore, nucleosomes are more physiologically relevant substrates than histones and histone-derived peptides for use in in vitro studies. More importantly, some histone methyltransferases are significantly more active, as well as specific, when using nucleosomal substrates in HMT assays, such as DOT1L and NSD family enzymes. Nucleosomes are also widely used in histone methyltransferase screening assays to identify small molecular inhibitors for drug discovery.
Background: In vivo, the nucleosome is the basic structural unit of chromatin. It is comprised of about 146 bp of DNA wrapped around a core of eight histones of four different types: H2A, H2B, H3 and H4. Histones are subject to post-translational modifications, such as methylation, acetylation, phosphorylation, mono-ubiquitination, etc. Histone modifications influence multiple chromatin templated processes such as gene transcription, DNA repair and recombination. Besides the "major" histones, there are some histone variants in specific regions of chromatin or in specific cell types. Histone variants are involved in multiple biological processes including chromosome segregation, DNA repair, transcriptional regulation and mRNA processing. H2A.Z (also known as H2AFZ, Histone Family Member Z) is a histone H2A family member. It is highly conserved from yeast to human, with 90% of its primary sequence preserved among different species, showing only 60% homology with canonical histone H2A. H2A.Z is found in approximately 10% of mammalian nucleosomes. H2A.Z has been one of the most studied H2A variants in recent years. It plays an important role in different biological processes such as DNA replication, DNA repair, transcription regulation, cell cycle, spermatogenesis, chromosome segregation, centromere structure and maintenance of heterochromatic/euchromatic status. However, different studies reported diverse conclusions in the nucleosome stability and transcriptional regulation. The contradictory roles of H2A.Z in vivo might be explained by different combinations of H2A.Z with other epigenetic regulators, PTMs on H2A.Z and interaction with chromatin binding proteins. Nucleosomes are more physiologically relevant substrates than histones and histone-derived peptides for in vitro studies. More importantly, some histone methyltransferases are significantly more active, as well as specific, when using nucleosomal substrates in HMT assays, such as DOT1L and NSD family enzymes. Nucleosomes are also widely used in histone methyltransferase screening assays to identify small molecular inhibitors for drug discovery.
Background: In vivo, the nucleosome is the basic structural unit of chromatin. It is comprised of about 146 bp of DNA wrapped around a core of eight histones of four different types: H2A, H2B, H3 and H4. Histones are subject to post-translational modifications, such as methylation, acetylation, phosphorylation, mono-ubiquitination, etc. Histone modifications influence multiple chromatin templated processes such as gene transcription, DNA repair and recombination. Besides the "major" histones, there are some histone variants in specific regions of chromatin or in specific cell types. Histone variants are involved in multiple biological processes including chromosome segregation, DNA repair, transcriptional regulation and mRNA processing. H2A.Z (also known as H2AFZ, Histone Family Member Z) is a histone H2A family member. It is highly conserved from yeast to human, with 90% of its primary sequence preserved among different species, showing only 60% homology with canonical histone H2A. H2A.Z is found in approximately 10% of mammalian nucleosomes. H2A.Z has been one of the most studied H2A variants in recent years. It plays an important role in different biological processes such as DNA replication, DNA repair, transcription regulation, cell cycle, spermatogenesis, chromosome segregation, centromere structure and maintenance of heterochromatic/euchromatic status. However, different studies reported diverse conclusions in the nucleosome stability and transcriptional regulation. The contradictory roles of H2A.Z in vivo might be explained by different combinations of H2A.Z with other epigenetic regulators, PTMs on H2A.Z and interaction with chromatin binding proteins. Nucleosomes are more physiologically relevant substrates than histones and histone-derived peptides for in vitro studies. More importantly, some histone methyltransferases are significantly more active, as well as specific, when using nucleosomal substrates in HMT assays, such as DOT1L and NSD family enzymes. Nucleosomes are also widely used in histone methyltransferase screening assays to identify small molecular inhibitors for drug discovery.
Product Categories/Family for H2AFZ recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13,553 Da
NCBI Official Full Name
histone H2A.Z
NCBI Official Synonym Full Names
H2A histone family, member Z
NCBI Official Symbol
H2AFZ
NCBI Official Synonym Symbols
H2AZ; H2A.z; H2A/z; H2A.Z-1
NCBI Protein Information
histone H2A.Z; H2AZ histone
UniProt Protein Name
Histone H2A.Z
UniProt Gene Name
H2AFZ
UniProt Synonym Gene Names
H2AZ; H2A/z
UniProt Entry Name
H2AZ_HUMAN
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Product Notes
The H2AFZ h2afz (Catalog #AAA59762) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Mononucleosomes, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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