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product-image-AAA120701_AD15.jpg Application Data (CALCULATION OF RESULTS)

Monkeypox Virus (MPXV) IgG ELISA Kit | H3L elisa kit

Human Anti-Monkeypox Virus (MPXV) IgG ELISA Kit (H3L)

Reactivity
Monkeypox Virus (MPXV)
Synonyms
Monkeypox Virus (MPXV) IgG; N/A; Human Anti-Monkeypox Virus (MPXV) IgG ELISA Kit (H3L); H3L; Monkeypox virus/MPXV; Mpox; H3L elisa kit
Ordering
Reactivity
Monkeypox Virus (MPXV)
Assay Type
Quantitative Competitive
Samples
Serum and plasma
Detection Range
469 ng/mL - 30,000 ng/mL
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): <20%. Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays): <20%. Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
Preparation and Storage
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong storage time, please store the Standard, Detection A, Detection B and Microplate at - 20 degree C, the rest reagents should be store at 4 degree C.

Application Data

(CALCULATION OF RESULTS)

product-image-AAA120701_AD15.jpg Application Data (CALCULATION OF RESULTS)
Related Product Information for H3L elisa kit
Principle of the Assay: This assay employs the quantitative competitive enzyme immunoassay technique. Recombinant Monkeypox virus H3L antigen has been pre-coated onto a microplate. Standards or samples are premixed with biotin-labeled antibody and then pipetted into the wells. Human Anti-Monkeypox virus IgG (H3L) in the sample competitively binds to the pre-coated protein with biotin-labeled Human Anti-Monkeypox virus IgG (H3L). After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in inversely proportion to the amount of Human Anti-Monkeypox virus IgG (H3L) bound in the initial step. The color development is stopped and the intensity of the color is measured.

Intended Uses: Used for the quantitative determination of Human Anti-Monkeypox virus IgG (H3L) concentration in serum and plasma.
Product Categories/Family for H3L elisa kit
References
Vaccinia virus H3L envelope protein is a major target of neutralizing antibodies in humans and elicits protection against lethal challenge in mice. PMID: 16140750
Poly[bis-[μ(2)-(dimethyl-aza-nium-yl)methyl-enediphospho-nato]magnesium]. PMID: 21522287
Vaccinia virus envelope H3L protein binds to cell surface heparan sulfate and is important for intracellular mature virion morphogenesis and virus infection in vitro and in vivo. PMID: 10708453
Cell population data: much more to explore. PMID: 36622868
Characterization of the vaccinia virus H3L envelope protein: topology and posttranslational membrane insertion via the C-terminal hydrophobic tail. PMID: 10906204

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Product Notes

The Monkeypox Virus (MPXV) H3L (Catalog #AAA120701) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA120701 ELISA Kit recognizes Monkeypox Virus (MPXV) H3L. It is sometimes possible for the material contained within the vial of "Monkeypox Virus (MPXV) IgG, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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