Application Data
(SDS-PAGE: purified HA (B/Florida/4/2006)(aa 16-546) proteinA, reducing condition; B, non-reducing condition)
HA (B/Florida/4/2006) Recombinant Protein | HA recombinant protein
HA (aa 16-546)(B/Florida/4/2006)
Applications
Western Blot, ELISA
Purity
> 95% purity (SDS-PAGE)
Synonyms
HA (B/Florida/4/2006); N/A; HA (aa 16-546)(B/Florida/4/2006); HA (B/Florida/4/2006)(aa 16-546); HA, 6xHis tagged ectodomain of human influenza B virus hemagglutinin (B/Florida/4/2006) (aa 17-546), glycosylated recombinant protein; HA recombinant protein
Host
293 cell culture
Purity/Purification
> 95% purity (SDS-PAGE)
Concentration
1 ug/ul in PBS (varies by lot)
Sequence
DRICTGITSSNSPHVVKTATQGEVNVTGVIPLTTTPTKSYFANLKGTRTRGKLCPDCLNCTDLDVALGRPMCVGTTPSAKASILHEVKPVTSGCFPIMHDRTKIRQLPNLLRGYENIRLSTQNVIDAKAPGGPYRLGTSGSCPNATSKSGFFATMAWAVPKDNNKNATNPLTVEVPYICTEGEDQITVWGFHSDDKTQMKNLYGDSNPQKFTSSANGVTTHYVSQIGSFPDQTEDGGLPQSGRIVVDYMMQKPGTGTIVYQRGVLLPQKVWCASGRSKVIKGSLPLIGEADCLHEKYGGLNKSKPYYTGEHAKAIGNCPIWVKTPLKLANGTKYRPPAKLLKERGFFGAIAGFLEGGWEGMIAGWHGYTSHGAHGVAVAADKSTQEAINKITKNLNSLSELEVKNLQRLSGAMDELHNEILELDEKVDDLRADTISSQIELAVLLSNEGIINSEDEHLLALERKLKKMLGPSAVEIGNGCFETKHKCNQTCLDRIAAGTFNAGEFSLPFDSLNITAASLNDDGLDNH
Sequence Length
584
Applicable Applications for HA recombinant protein
Western Blot (WB), ELISA (EIA), Immunogen
Application Notes
Western blot standard, antibody ELISA, antigen, etc.
Endotoxin Level
<0.01 EU per 1 ug of the protein by LAL test
Viral Protein
C-terminal 6xHis tagged ectodomain of human influenza B virus (B/Florida/4/2006) HA protein (amino acid 16-546) (Genebank No. ACA33493)
Preparation and Storage
Store at -20 degrees C; Stable for 6-months from the date of receipt when kept at 4 degrees C. Non-hazardous. No MSDS required
Application Data
(SDS-PAGE: purified HA (B/Florida/4/2006)(aa 16-546) proteinA, reducing condition; B, non-reducing condition)
Application Data
(SDS-PAGE: purified HA (B/Florida/4/2006)(aa 16-546) proteinA, reducing condition; B, non-reducing condition)
Related Product Information for HA recombinant protein
Description:Viral protein purified from 293 cell culture
Introduction: Influenza hemagglutinin (HA) is a type of hemagglutinin found on the surface of the influenza viruses. HA is an antigenic glycoprotein, like all other hemagglutinins, it causes red blood cells to agglutinate. HA is responsible for binding the virus to the cell that is being infected. HA proteins bind to cells with sialic acid on the membranes, such as cells in the upper respiratory tract or erythrocytes.
HA is a homotrimeric integral membrane glycoprotein. HA monomer is synthesized as a single polypeptide that is subsequently cleaved into two smaller polypeptides, the HA1 and HA2 subunits. Each HA monomer consists of a long, helical chain anchored in the membrane by HA2 and topped by a large HA1 globule.
Introduction: Influenza hemagglutinin (HA) is a type of hemagglutinin found on the surface of the influenza viruses. HA is an antigenic glycoprotein, like all other hemagglutinins, it causes red blood cells to agglutinate. HA is responsible for binding the virus to the cell that is being infected. HA proteins bind to cells with sialic acid on the membranes, such as cells in the upper respiratory tract or erythrocytes.
HA is a homotrimeric integral membrane glycoprotein. HA monomer is synthesized as a single polypeptide that is subsequently cleaved into two smaller polypeptides, the HA1 and HA2 subunits. Each HA monomer consists of a long, helical chain anchored in the membrane by HA2 and topped by a large HA1 globule.
Product Categories/Family for HA recombinant protein
References
Velkov T, etc. A gel-capture assay for characterizing the sialys-glycan selectivity of influenza viruses. Acta Virol. 55, 131-137, 2011.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
hemagglutinin
UniProt Protein Name
Hemagglutinin
UniProt Gene Name
HA
UniProt Entry Name
B1AG46_9INFB
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Product Notes
The HA ha (Catalog #AAA13746) is a Recombinant Protein produced from 293 cell culture and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HA (B/Florida/4/2006) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunogen. Western blot standard, antibody ELISA, antigen, etc. . Researchers should empirically determine the suitability of the HA ha for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DRICTGITSS NSPHVVKTAT QGEVNVTGVI PLTTTPTKSY FANLKGTRTR GKLCPDCLNC TDLDVALGRP MCVGTTPSAK ASILHEVKPV TSGCFPIMHD RTKIRQLPNL LRGYENIRLS TQNVIDAKAP GGPYRLGTSG SCPNATSKSG FFATMAWAVP KDNNKNATNP LTVEVPYICT EGEDQITVWG FHSDDKTQMK NLYGDSNPQK FTSSANGVTT HYVSQIGSFP DQTEDGGLPQ SGRIVVDYMM QKPGTGTIVY QRGVLLPQKV WCASGRSKVI KGSLPLIGEA DCLHEKYGGL NKSKPYYTGE HAKAIGNCPI WVKTPLKLAN GTKYRPPAKL LKERGFFGAI AGFLEGGWEG MIAGWHGYTS HGAHGVAVAA DKSTQEAINK ITKNLNSLSE LEVKNLQRLS GAMDELHNEI LELDEKVDDL RADTISSQIE LAVLLSNEGI INSEDEHLLA LERKLKKMLG PSAVEIGNGC FETKHKCNQT CLDRIAAGTF NAGEFSLPFD SLNITAASLN DDGLDNH. It is sometimes possible for the material contained within the vial of "HA (B/Florida/4/2006), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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