SDS-PAGE
(Reduced SDS-PAGE: purified HA (aa 18-518)(A/Puerto Rico/8/1934)(H1N1) protein)
HA recombinant protein
HA (A/Puerto Rico/8/1934)(H1N1)(aa 18-518) Trimer
Purity
>95% pure by 4-20% SDS-PAGE gel
Synonyms
HA; N/A; HA (A/Puerto Rico/8/1934)(H1N1)(aa 18-518) Trimer; HA recombinant protein
Host
293 cell culture
Purity/Purification
>95% pure by 4-20% SDS-PAGE gel
Concentration
1 ug/ul in PBS (varies by lot)
Sequence
DTICIGYHANNSTDTVDTVLEKNVTVTHSVNLLEDSHNGKLCRLKGIAPLQLGKCNIAGWLLGNPECDPLLPVRSWSYIVETPNSENGICYPGDFIDYEELREQLSSVSSFERFEIFPKESSWPNHNTNGVTAACSHEGKSSFYRNLLWLTEKEGSYPKLKNSYVNKKGKEVLVLWGIHHPPNSKEQQNLYQNENAYVSVVTSNYNRRFTPEIAERPKVRDQAGRMNYYWTLLKPGDTIIFEANGNLIAPMYAFALSRGFGSGIITSNASMHECNTKCQTPLGAINSSLPYQNIHPVTIGECPKYVRSAKLRMVTGLRNIPSIQSRGLFGAIAGFIEGGWTGMIDGWYGYHHQNEQGSGYAADQKSTQNAINGITNKVNTVIEKMNIQFTAVGKEFNKLEKRMENLNKKVDDGFLDIWTYNAELLVLLENERTLDFHDSNVKNLYEKVKSQLKNNAKEIGNGCFEFYHKCDNECMESVRNGTYDYPKYSEESKLNREKVDG
Application Notes
Western Blot (WB), ELISA (EIA), Antigen
Endotoxin
<0.01 EU per 1 ug of the protein by LAL test
Viral Protein
C-terminal 6xHis tagged hemagglutinin (amino acid 18-518) (H1N1)(A/Puerto Rico/8/1934) protein (GenBank Accession No. AB671289.1). A trimerization domain sequence has been introduced into the C-terminal of HA to stabilize the formation of trimer HA.
Preparation and Storage
Store at -20°C; Stable for 6-months from the date of receipt when kept at 4°C. Nonhazardous. No MSDS required.
SDS-PAGE
(Reduced SDS-PAGE: purified HA (aa 18-518)(A/Puerto Rico/8/1934)(H1N1) protein)
SDS-PAGE
(Reduced SDS-PAGE: purified HA (aa 18-518)(A/Puerto Rico/8/1934)(H1N1) protein)
Related Product Information for HA recombinant protein
Introduction: Influenza hemagglutinin (HA) is a type of hemagglutinin found on the surface of the influenza viruses. HA is an antigenic glycoprotein, like all other hemagglutinins, it causes red blood cells to agglutinate. HA is responsible for binding the virus to the cell that is being infected. HA proteins bind to cells with sialic acid on the membranes, such as cells in the upper respiratory tract or erythrocytes.
HA is a homotrimeric integral membrane glycoprotein. HA monomer is synthesized as a single polypeptide that is subsequently cleaved into two smaller polypeptides, the HA 1 and HA2 subunits. Each HA monomer consists of a long, helical chain anchored in the membrane by HA2 and topped by a large HA 1 globule.
Description: Viral protein purified from 293 cell culture
HA is a homotrimeric integral membrane glycoprotein. HA monomer is synthesized as a single polypeptide that is subsequently cleaved into two smaller polypeptides, the HA 1 and HA2 subunits. Each HA monomer consists of a long, helical chain anchored in the membrane by HA2 and topped by a large HA 1 globule.
Description: Viral protein purified from 293 cell culture
Product Categories/Family for HA recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
HA, partial
UniProt Protein Name
Hemagglutinin
UniProt Gene Name
HA
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Product Notes
The HA ha (Catalog #AAA13698) is a Recombinant Protein produced from 293 cell culture and is intended for research purposes only. The product is available for immediate purchase. Western Blot (WB), ELISA (EIA), Antigen. Researchers should empirically determine the suitability of the HA ha for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DTICIGYHAN NSTDTVDTVL EKNVTVTHSV NLLEDSHNGK LCRLKGIAPL QLGKCNIAGW LLGNPECDPL LPVRSWSYIV ETPNSENGIC YPGDFIDYEE LREQLSSVSS FERFEIFPKE SSWPNHNTNG VTAACSHEGK SSFYRNLLWL TEKEGSYPKL KNSYVNKKGK EVLVLWGIHH PPNSKEQQNL YQNENAYVSV VTSNYNRRFT PEIAERPKVR DQAGRMNYYW TLLKPGDTII FEANGNLIAP MYAFALSRGF GSGIITSNAS MHECNTKCQT PLGAINSSLP YQNIHPVTIG ECPKYVRSAK LRMVTGLRNI PSIQSRGLFG AIAGFIEGGW TGMIDGWYGY HHQNEQGSGY AADQKSTQNA INGITNKVNT VIEKMNIQFT AVGKEFNKLE KRMENLNKKV DDGFLDIWTY NAELLVLLEN ERTLDFHDSN VKNLYEKVKS QLKNNAKEIG NGCFEFYHKC DNECMESVRN GTYDYPKYSE ESKLNREKVD G. It is sometimes possible for the material contained within the vial of "HA, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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