Bioactivity
(Hemoglobin (HB) is a protein in red blood cells which contains iron. It is used to transport oxygen around the human body. Hemoglobin is found in the red blood cells of almost all vertebrates. Hemoglobin has peroxidase activity. In the presence of hydrogen peroxide, hemoglobin can catalyze the substrates 3,3 ', 5,5' - tetramethylbenzidine (TMB) to produce a blue compound which has a maximum absorption at 595 nm. Thus, the activity of native chinese hamster hemoglobin was measured by its ability to catalyze the substrates of TMB. The reaction was performed in PBS, pH 7.4 (Assay Buffer), ainitiated by addition 40 uL of various concentrations of HB (diluted by Assay Buffer) to 160 uL of TMB. Incubated at 37 degree C for 20min, then read at a wavelength of 595 nm immediately. The result was shown in figure 1, and the OD value was in a linear relationship with the concentration of HB.)
Hemoglobin (HB) Active Protein | HB active protein
Active Hemoglobin (HB)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Synonyms
Hemoglobin (HB); N/A; Active Hemoglobin (HB); Hemoglobin; Hgb; Haemoglobin; Heterotetramer(alphabeta)24; HB active protein
Host
Hamster
Purity/Purification
Greater than 90% by SDS-PAGE
Form/Format
Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Applicable Applications for HB active protein
Activity Assay, Cell Culture
Species
Chinese hamster
Expression System
Natural extract
Tag
N-terminal His Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Research Area
Metabolic pathway, Infection immunity, Hematology, Neuro science
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Bioactivity
(Hemoglobin (HB) is a protein in red blood cells which contains iron. It is used to transport oxygen around the human body. Hemoglobin is found in the red blood cells of almost all vertebrates. Hemoglobin has peroxidase activity. In the presence of hydrogen peroxide, hemoglobin can catalyze the substrates 3,3 ', 5,5' - tetramethylbenzidine (TMB) to produce a blue compound which has a maximum absorption at 595 nm. Thus, the activity of native chinese hamster hemoglobin was measured by its ability to catalyze the substrates of TMB. The reaction was performed in PBS, pH 7.4 (Assay Buffer), ainitiated by addition 40 uL of various concentrations of HB (diluted by Assay Buffer) to 160 uL of TMB. Incubated at 37 degree C for 20min, then read at a wavelength of 595 nm immediately. The result was shown in figure 1, and the OD value was in a linear relationship with the concentration of HB.)
Bioactivity
(Hemoglobin (HB) is a protein in red blood cells which contains iron. It is used to transport oxygen around the human body. Hemoglobin is found in the red blood cells of almost all vertebrates. Hemoglobin has peroxidase activity. In the presence of hydrogen peroxide, hemoglobin can catalyze the substrates 3,3 ', 5,5' - tetramethylbenzidine (TMB) to produce a blue compound which has a maximum absorption at 595 nm. Thus, the activity of native chinese hamster hemoglobin was measured by its ability to catalyze the substrates of TMB. The reaction was performed in PBS, pH 7.4 (Assay Buffer), ainitiated by addition 40 uL of various concentrations of HB (diluted by Assay Buffer) to 160 uL of TMB. Incubated at 37 degree C for 20min, then read at a wavelength of 595 nm immediately. The result was shown in figure 1, and the OD value was in a linear relationship with the concentration of HB.)
SDS-PAGE
(Figure. SDS-PAGE)
SDS-PAGE
(Figure. SDS-PAGE)
Product Categories/Family for HB active protein
NCBI and Uniprot Product Information
Similar Products
Product Notes
The HB hba (Catalog #AAA161849) is an Active Protein produced from Hamster and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Hemoglobin (HB) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the HB hba for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hemoglobin (HB), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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