Application Data
(HTRF assay for HDAC3 / NCOR2 complex activity 3 uM Histone H3K9ac (1-21aa) peptide was incubated with different concentrations of HDAC3 / NCOR2 complex in a reaction system with 25 mM Tris-HCl pH 8, 137 mM NaCl, 2.7 mM KCl, 1mM MgCl2 and 0.1 mg/ml BSA for 30 min at 37 degree C, then H3K9me0 antibody and SA-XL665 mixture was added to each for 30 min. Operations were performed at room temp. HTRF assay was used for detection.)
HDAC3/NCOR2 recombinant protein
Recombinant HDAC3/NCOR2 complex
Gene Names
HDAC3; HD3; RPD3; RPD3-2
Purity
The purity of HDAC3/NCOR2 complex is >90% by SDS-PAGE.
Synonyms
HDAC3/NCOR2; N/A; Recombinant HDAC3/NCOR2 complex; Histone Deacetylase 3; HD3; SMAP45; HDAC3/NCOR2 recombinant protein
Host
Baculovirus
Purity/Purification
The purity of HDAC3/NCOR2 complex is >90% by SDS-PAGE.
Form/Format
Recombinant HDAC3/NCOR2 complex is supplied in 25mM HEPES pH7.5, 300mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.5mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag, His-Tag
Notes
This HDAC3/NCOR2 protein complex is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(HTRF assay for HDAC3 / NCOR2 complex activity 3 uM Histone H3K9ac (1-21aa) peptide was incubated with different concentrations of HDAC3 / NCOR2 complex in a reaction system with 25 mM Tris-HCl pH 8, 137 mM NaCl, 2.7 mM KCl, 1mM MgCl2 and 0.1 mg/ml BSA for 30 min at 37 degree C, then H3K9me0 antibody and SA-XL665 mixture was added to each for 30 min. Operations were performed at room temp. HTRF assay was used for detection.)
Application Data
(HTRF assay for HDAC3 / NCOR2 complex activity 3 uM Histone H3K9ac (1-21aa) peptide was incubated with different concentrations of HDAC3 / NCOR2 complex in a reaction system with 25 mM Tris-HCl pH 8, 137 mM NaCl, 2.7 mM KCl, 1mM MgCl2 and 0.1 mg/ml BSA for 30 min at 37 degree C, then H3K9me0 antibody and SA-XL665 mixture was added to each for 30 min. Operations were performed at room temp. HTRF assay was used for detection.)
Application Data
(HTRF assay for HDAC3 / NCOR2 complex activity 3 uM Histone H3K9ac (1-21aa) peptide was incubated with different concentrations of HDAC3 / NCOR2 complex in a reaction system with 25 mM Tris-HCl pH 8, 137 mM NaCl, 2.7 mM KCl, 1mM MgCl2 and 0.1 mg/ml BSA for 30 min at 37 degree C, then H3K9me0 antibody and SA-XL665 mixture was added to each for 30 min. Operations were performed at room temp. HTRF assay was used for detection.)
Application Data
(HTRF assay for HDAC3 / NCOR2 complex activity 3 uM Histone H3K9ac (1-21aa) peptide was incubated with different concentrations of HDAC3 / NCOR2 complex in a reaction system with 25 mM Tris-HCl pH 8, 137 mM NaCl, 2.7 mM KCl, 1mM MgCl2 and 0.1 mg/ml BSA for 30 min at 37 degree C, then H3K9me0 antibody and SA-XL665 mixture was added to each for 30 min. Operations were performed at room temp. HTRF assay was used for detection.)
SDS-PAGE
(Recombinant HDAC3 / NCOR2 complex gel. HDAC3 / NCOR2 complex was run on an 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW of HDAC3: 50.8 kDa MW of NCOR2: 14.6 kDa)
SDS-PAGE
(Recombinant HDAC3 / NCOR2 complex gel. HDAC3 / NCOR2 complex was run on an 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW of HDAC3: 50.8 kDa MW of NCOR2: 14.6 kDa)
Related Product Information for HDAC3/NCOR2 recombinant protein
Short Description: Recombinant HDAC3/NCOR2 complex includes full-length human HDAC3 protein (accession number NP_003874.2) with a C-terminal DYKDDDDK-Tag and human NCOR2 amino acids 395-489 (accession number NP_006303.4) with an N-terminal 6xHis-tag; it was expressed in Sf9 cells. The molecular weights of HDAC3 and NCOR2 (395-489) are 50.8 kDa and 14.6 kDa, respectively. The purity of HDAC3/NCOR2 complex is >90% by SDS-PAGE. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: HDAC3 (Histone Deacetylase 3, also designated HD3) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and 10), class III (SIRT1-7) and class IV (HDAC11). By forming multi-protein complexes with the co-repressor NCOR2, HDAC3 regulates the transcription of a plethora of genes. A growing list of non-histone proteins extends the role of HDAC3 beyond transcriptional repression. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both H3 and H4 in free histones or nucleosome substrate.
Background: HDAC3 (Histone Deacetylase 3, also designated HD3) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and 10), class III (SIRT1-7) and class IV (HDAC11). By forming multi-protein complexes with the co-repressor NCOR2, HDAC3 regulates the transcription of a plethora of genes. A growing list of non-histone proteins extends the role of HDAC3 beyond transcriptional repression. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both H3 and H4 in free histones or nucleosome substrate.
Product Categories/Family for HDAC3/NCOR2 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,848 Da
NCBI Official Full Name
histone deacetylase 3
NCBI Official Synonym Full Names
histone deacetylase 3
NCBI Official Symbol
HDAC3
NCBI Official Synonym Symbols
HD3; RPD3; RPD3-2
NCBI Protein Information
histone deacetylase 3; SMAP45
UniProt Protein Name
Histone deacetylase 3
UniProt Gene Name
HDAC3
UniProt Synonym Gene Names
HD3
UniProt Entry Name
HDAC3_HUMAN
Similar Products
Product Notes
The HDAC3/NCOR2 hdac3 (Catalog #AAA59734) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "HDAC3/NCOR2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.