Application Data
(HDAC-Glo Class IIa Assay for HDAC4 (627-1084) activity. Assay was performed using HDAC-Glo Class IIa Assay from Promega. 3.5 ?M substrate was incubated with HDAC4 (627-1084) proteins and 1/20,000 developer reagent at room temperature, then luminescence was detected after incubation for 20 min.)
HDAC4 recombinant protein
Recombinant HDAC4 (627-1084) protein
Gene Names
HDAC4; HD4; AHO3; BDMR; HDACA; HA6116; HDAC-4; HDAC-A
Synonyms
HDAC4; N/A; Recombinant HDAC4 (627-1084) protein; Histone Deacetylase 4; HD4; Histone Deactylase A; HDAC-4; HDACA; EC 3.5.1.98; HDAC4 recombinant protein
Host
Baculovirus
Form/Format
Recombinant HDAC4 (627-1084) protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Notes
Recombinant HDAC4 protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(HDAC-Glo Class IIa Assay for HDAC4 (627-1084) activity. Assay was performed using HDAC-Glo Class IIa Assay from Promega. 3.5 ?M substrate was incubated with HDAC4 (627-1084) proteins and 1/20,000 developer reagent at room temperature, then luminescence was detected after incubation for 20 min.)
Application Data
(HDAC-Glo Class IIa Assay for HDAC4 (627-1084) activity. Assay was performed using HDAC-Glo Class IIa Assay from Promega. 3.5 ?M substrate was incubated with HDAC4 (627-1084) proteins and 1/20,000 developer reagent at room temperature, then luminescence was detected after incubation for 20 min.)
Application Data
(HDAC-Glo Class IIa Assay for HDAC4 (627-1084) activity. Assay was performed using HDAC-Glo Class IIa Assay from Promega. 3.5 ?M substrate was incubated with HDAC4 (627-1084) proteins and 1/20,000 developer reagent at room temperature, then luminescence was detected after incubation for 20 min.)
Application Data
(HDAC-Glo Class IIa Assay for HDAC4 (627-1084) activity. Assay was performed using HDAC-Glo Class IIa Assay from Promega. 3.5 ?M substrate was incubated with HDAC4 (627-1084) proteins and 1/20,000 developer reagent at room temperature, then luminescence was detected after incubation for 20 min.)
SDS-PAGE
(Recombinant HDAC4 (627-1084) protein gel. HDAC4 (627-1084) protein was run on a 10% SDS- PAGE gel and stained with Coomassie Blue. MW: 52.9 kDa : Purity >90%)
SDS-PAGE
(Recombinant HDAC4 (627-1084) protein gel. HDAC4 (627-1084) protein was run on a 10% SDS- PAGE gel and stained with Coomassie Blue. MW: 52.9 kDa : Purity >90%)
Related Product Information for HDAC4 recombinant protein
Short Description: Recombinant HDAC4 (627-1084) protein includes amino acids 627-1084 of human HDAC4 protein (accession number NP_006028.2); it was expressed in Sf9 cells and contains an N-terminal DYKDDDDK-Tag with a molecular weight of 52.9 kDa. The purity of HDAC4 protein is >90% by SDS-PAGE. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: HDAC4 (Histone Deacetylase 4) is a member of the class IIa mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue..In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11). Unlike other deacetylases, HDAC4 shuttles between the nucleus and cytoplasm and serves as a nuclear co-repressor that regulates bone and muscle development. HDAC4 interacts with the myocyte enhancer factors Mef2a, Mef2c and Mef2d. It also forms part of a multi-protein complex with RbAp48 and HDAC3. HDAC4 is ubiquitous.
Background: HDAC4 (Histone Deacetylase 4) is a member of the class IIa mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue..In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11). Unlike other deacetylases, HDAC4 shuttles between the nucleus and cytoplasm and serves as a nuclear co-repressor that regulates bone and muscle development. HDAC4 interacts with the myocyte enhancer factors Mef2a, Mef2c and Mef2d. It also forms part of a multi-protein complex with RbAp48 and HDAC3. HDAC4 is ubiquitous.
Product Categories/Family for HDAC4 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
119,040 Da
NCBI Official Full Name
histone deacetylase 4
NCBI Official Synonym Full Names
histone deacetylase 4
NCBI Official Symbol
HDAC4
NCBI Official Synonym Symbols
HD4; AHO3; BDMR; HDACA; HA6116; HDAC-4; HDAC-A
NCBI Protein Information
histone deacetylase 4; histone deacetylase A
UniProt Protein Name
Histone deacetylase 4
UniProt Gene Name
HDAC4
UniProt Synonym Gene Names
KIAA0288; HD4
UniProt Entry Name
HDAC4_HUMAN
Similar Products
Product Notes
The HDAC4 hdac4 (Catalog #AAA59735) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "HDAC4, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.