HDAC6 recombinant protein

Recombinant HDAC6 (597-728, deleted mutant) protein

Cat. Num. AAA59727

• Gene Names: HDAC6; HD6; JM21
• Purity: The recombinant protein is >80% pure by SDS-PAGE.
• Synonyms: HDAC6; N/A; Recombinant HDAC6 (597-728, deleted mutant) protein; HD6; JM21; CPBHM; PPP1R90; HDAC6 recombinant protein

• Host: Baculovirus
• Purity/Purification: The recombinant protein is >80% pure by SDS-PAGE.
• Form/Format: Recombinant HDAC6 (597-728, deleted mutant) is supplied in 25mM Hepes pH7.5, 300mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2mM TCEP, 0.2mg/ml 3× DYKDDDDK peptide.

• Protein Species: Human
• Tag: DYKDDDDK-Tag, His-Tag
• Notes: Recombinant HDAC6 (597-728, deleted mutant) protein is suitable for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. HDAC Activity Assay Conditions: 3uM H3K9ac (1-21) peptides was incubated with different concentrations of HDAC6 (597-728, deleted mutant) in 25mM Tris-HCl, pH 8.0, 137mM NaCl, 2.7mM KCl, 1mM MgCl2, 0.1mg/ml BSA for 30 minutes at 37 degree C followed by developing for 30 minutes at room temperature.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

Application Data

(Recombinant HDAC6 (597-728, deleted mutant) protein activity assay. 3 ?M H3K9ac (1-21) peptides was incubated with different concentrations of HDAC6 (597-728, deleted mutant) in reaction buffer for 30 minutes at 37 degree C followed by developing for 30 minutes at room temperature.)

SDS-PAGE

(Recombinant HDAC6 (597-728, deleted mutant) protein gel. HDAC6 protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.)

Related Product Information for HDAC6 recombinant protein

Short Description: Recombinant HDAC6 (597-728, deleted mutant) deletes the second catalytic domain. This recombinant protein was expressed in a baculovirus expression system (accession number NP_006035.2) with an N-terminal 6xHis-tag and DYKDDDDK-Tag. The molecular weight of the protein is 122.1 kDa. The recombinant protein is >80% pure by SDS-PAGE. It is suitable for use in histone deacetylase (HDAC) assays, in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Background: HDAC6 (Histone Deacetylase 6) is a member of the class IIb mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones or many cellular proteins. Lysine N-epsilon-acetylation is a dynamic, reversible and tightly regulatedprotein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the e-amino group of a lysine residue. In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11). HDAC6 is a unique enzyme that harbors a full duplication of its deacetylase homology region, which appears to contribute independently to the overall activity of HDAC6 protein. Alpha-tubulin and HSP90 are two known substrates of HDAC6, playing an important role in cellular mechanisms related to the microtubule network and HSP90-dependent events. HDAC6 also participates in regulating expression of a group of genes involved in the remodeling of chromatin during cell differentiation.

Product Categories/Family for HDAC6 recombinant protein

Recombinant Proteins; Histone Deacetylase (HDAC)

NCBI and Uniprot Product Information

• NCBI GI #: 13128864
• NCBI GeneID: 10013
• NCBI Accession #: NP_006035.2
• NCBI GenBank Nucleotide #: NM_006044.2
• UniProt Accession #: Q9UBN7; O94975; Q6NT75; Q7L3E5; Q96CY0
• Molecular Weight: 131,419 Da
• NCBI Official Full Name: histone deacetylase 6
• NCBI Official Synonym Full Names: histone deacetylase 6
• NCBI Official Symbol: HDAC6
• NCBI Official Synonym Symbols: HD6; JM21
• NCBI Protein Information: histone deacetylase 6
• UniProt Protein Name: Histone deacetylase 6
• UniProt Gene Name: HDAC6
• UniProt Synonym Gene Names: KIAA0901; HD6
• UniProt Entry Name: HDAC6_HUMAN