Betacellulin/BTC Active Protein | BTC active protein

Recombinant human Betacellulin/BTC Protein

Cat. Num. AAA48393

• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: Betacellulin/BTC; N/A; Recombinant human Betacellulin/BTC Protein; Betacellulin; Probetacellulin; BTC; Betacellulin isoform 1; BTC active protein

• Host: HEK293
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol.
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 32-111aa
• Sequence: DGNSTRSPET NGLLCGDPEE NCAATTTQSK RKGHFSRCPK QYKHYCIKGR CRFVVAEQTP SCVCDEGYIG ARCERVDLFY

• Applicable Applications for BTC active protein: Bioactivity, SDS-PAGE
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Human
• Tag: His-Tag
• Predicted N-term: Asp32
• Bioactivity: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 range <= 0.5ng/ml.
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

Bioactivity

(Human BTC stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cells. The ED50 range )

SDS-PAGE

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for BTC active protein

BTC, also known as Betacellulin, is a member of epidermal growth factor receptor family and functions as a ligand for the epidermal growth factor receptor (EGFR). As the role a EGFR, betacellulin is manifested by different form of muscles and tissues, it also has a great effect of nitrogen that is used for retinal pigment epithelial cells and vascular smooth muscle cells. While many studies attest a role for betacellulin in the differentiation of pancreatic beta-cells, the last decade witnessed the association of betacellulin with many additional biological processes, ranging from reproduction to the control of neural stem cells. As a typical EGFR ligand, betacellulin is expressed by a variety of cell types and tissues, and the soluble growth factor is proteolytically cleaved from a larger membrane-anchored precursor. It stimulates the proliferation of retinal pigment epithelial and vascular smooth muscle cells but did not stimulate the growth of several other cell types, such as endothelial cells and fetal lung fibroblasts. Recombinant human Betacellulin, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Categories/Family for BTC active protein

Signal Transduction; Proteins

References

Saito T., et al, (2004) Endocrinology. 145(9):4232-4243. Kim HS., et al, (2003) FASEB Journal. 17(2):318-320.

NCBI and Uniprot Product Information

• NCBI GI #: 4502461
• NCBI GeneID: 685
• NCBI Accession #: NP_001720
• NCBI GenBank Nucleotide #: NM_001729.3
• UniProt Accession #: P35070; Q96F48
• Molecular Weight: 11.3kD (101aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
• NCBI Official Full Name: probetacellulin isoform 1 preproprotein
• NCBI Official Synonym Full Names: betacellulin
• NCBI Official Symbol: BTC
• NCBI Protein Information: probetacellulin
• UniProt Protein Name: Probetacellulin
• UniProt Gene Name: BTC
• UniProt Synonym Gene Names: BTC
• UniProt Entry Name: BTC_HUMAN