EphA3 active protein

Recombinant mouse EphA3 protein

Cat. Num. AAA48999

• Gene Names: Epha3; Hek; Cek4; ETK1; End3; Hek4; Mek4; Tyro4; AW492086
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: EphA3; N/A; Recombinant mouse EphA3 protein; ephrin type-A receptor 3; ephrin type-A receptor 3 isoform1; receptor protein-tyrosine kinase; EPH-like kinase 4; tyrosine-protein kinase TYRO4; tyrosine-protein kinase receptor ETK1; EpHreceptor A3; AW492086; Cek4; EK4; End3; ETK1; Hek; Hek4; Mek4; Tyro4; EphA3 active protein

• Host: HEK293
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid in. Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 1mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 21-541aa
• Sequence: Glu 21; ELSPQPSNEV NLLDSKTIQG ELGWISYPSH GWEEISGVDE HYTPIRTYQV CNVMDHSQNN WLRTNWVPRN SAQKIYVELK FTLRDCNSIP LVLGTCKETF NLYYMESDDD HGVKFREHQF TKIDTIAADE SFTQMDLGDR ILKLNTEIRE VGPVNKKGFY LAFQDVGACV ALVSVRVYFK KCPFTVKNLA MFPDTVPMDS QSLVEVRGSC VNNSKEEDPP RMYCSTEGEW LVPIGKCTCN AGYEERGFIC QACRPGFYKA SDGAAKCAKC PPHSSTQEDG SMNCRCENNY FRAEKDPPSM ACTRPPSAPR NVISNINETS VILDWSWPLD TGGRKDITFN IICKKCGWNV RQCEPCSPNV RFLPRQLGLT NTTVTVTDLL AHTNYTFEID AVNGVSELSS PPRQYAAVSI TTNQAAPSPV MTIKKDRTSR NSISLSWQEP EHPNGIILDY EVKYYEKQEQ ETSYTILRAR GTNVTISSLK PDTTYVFQIR ARTAAGYGTN SRKFEFETSP DSFSISGENS H

• Applicable Applications for EphA3 active protein: Bioactivity, SDS-PAGE
• Species: Mouse
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
• Tag: His-Tag
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Related Product Information for EphA3 active protein

EphA3 is a protein-tyrosine kinase that belongs to the ephrin receptor subfamily. It has an extracellular region with a Cys-rich domain and two fibronectin type III repeats, and a single kinase domain. EpHand other EPH-related receptors are involved in various developmental processes, especially in the nervous system. EphA3 interacts with EFNB2 and EFNA5, and is expressed in the forebrain, retinal axons, some motor neurons in the spinal cord, and the heart during development. It regulates axonal guidance and organ formation. It is also found on some blood and solid tumor cells, and on astrocytes near injured nerve tissue. Recombinant mouse EphA3, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Categories/Family for EphA3 active protein

Angiogenesis, Cardiovascular; Proteins

References

Kudo, C. et al. (2005) J. Comp. Neurol. 487:255-269.
Kilpatrick, T.J. et al. (1996) Mol. Cell. Neurosci. 7:62-74.
Stephen, L.J. et al. (2007) Dev. Biol. 302:66-79.
Merlos-Suarez, A. and E. Batlle (2008) Curr. Opin. Cell Biol. 20:194-200.

NCBI and Uniprot Product Information

• NCBI GI #: 31982448
• NCBI GeneID: 13837
• NCBI Accession #: NP_034270.1
• NCBI GenBank Nucleotide #: NM_010140.3
• UniProt Accession #: Q8BRB1; P29319
• Molecular Weight: 109,955 Da
• NCBI Official Full Name: ephrin type-A receptor 3
• NCBI Official Synonym Full Names: Eph receptor A3
• NCBI Official Symbol: Epha3
• NCBI Official Synonym Symbols: Hek; Cek4; ETK1; End3; Hek4; Mek4; Tyro4; AW492086
• NCBI Protein Information: ephrin type-A receptor 3; EK4; EPH-like kinase 4; tyrosine-protein kinase TYRO4; tyrosine-protein kinase receptor ETK1
• UniProt Protein Name: Eph receptor A3
• UniProt Gene Name: Epha3
• UniProt Entry Name: Q8BRB1_MOUSE