EphB1 active protein

Recombinant human EphB1 protein

Cat. Num. AAA48404

• Gene Names: EPHB1; ELK; NET; Hek6; EPHT2
• Applications: SDS-Page
• Purity: >95% by SDS-PAGE
• Synonyms: EphB1; N/A; Recombinant human EphB1 protein; EPHB-1; Ephrin type-B receptor 1; Eph Receptor B1; ELK; EPH tyrosine kinase 2; EPH-like kinase 6; EK6; hEK6; HEK6; Neuronally-expressed EPH-related tyrosine kinase; NET; NETHek6; Tyrosine-protein kinase receptor EPH-2; EPHT2; soluble EPHB1 variant 1; EphB1 active protein

• Host: HEK293
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 0.25mg/ml (determined by absorbance at 280nm) (varies by lot)
• Sequence Positions: 18-540aa
• Sequence: MEETLMDTRT ATAELGWTAN PASGWEEVSG YDENLNTIRT YQVCNVFEPN QNNWLLTTFI NRRGAHRIYT EMRFTVRDCS SLPNVPGSCK ETFNLYYYET DSVIATKKSA FWSEAPYLKV DTIAADESFS QVDFGGRLMK VNTEVRSFGP LTRNGFYLAF QDYGACMSLL SVRVFFKKCP SIVQNFAVFP ETMTGAESTS LVIARGTCIP NAEEVDVPIK LYCNGDGEWM VPIGRCTCKP GYEPENSVAC KACPAGTFKA SQEAEGCSHC PSNSRSPAEA SPICTCRTGY YRADFDPPEV ACTSVPSGPR NVISIVNETS IILEWHPPRE TGGRDDVTYN IICKKCRADR RSCSRCDDNV EFVPRQLGLT ECRVSISSLW AHTPYTFDIQ AINGVSSKSP FPPQHVSVNI TTNQAAPSTV PIMHQVSATM RSITLSWPQP EQPNGIILDY EIRYYEKEHN EFNSSMARSQ TNTARIDGLR PGMVYVVQVR ARTVAGYGKF SGKMCFQTLT DDDYKSELRE QLP

• Applicable Applications for EphB1 active protein: Bioactivity, SDS-PAGE
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Human
• Tag: His-Tag
• Predicted N-term: Met18
• Bioactivity: Measured by its binding ability in a functional ELISA with Human EFNB1.
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

Bioactivity

(Human EPHB1 is coated at 2 ug/ml (100 ul/well) can bind Human EFNB1 (CAT# ATGP3800) in a Functional ELISA assay.)

SDS-PAGE

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for EphB1 active protein

EphB1 also known as Ephrin type-B receptor 1, is a member of the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors. EphB1 has been shown to bind ephrin-B2, ephrin-B1,ephrin?A3, ephrin-A1, ephrin-A4, and ephrin-B3. It binds to tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. Ephrin receptors and their ligands, the  ephrins, mediate numerous developmental processes, particularly in the nervous system. The ephrin/Eph families also appear to play a role in angiogenesis. Recombinant human EPHB1, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Categories/Family for EphB1 active protein

Angiogenesis; Cardiovascular; Proteins

References

Eph Nomenclature Committee. Cell 90:403-404. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608-615. Adams RH, et al. (1999). Genes Dev. 13: 295-306.

NCBI and Uniprot Product Information

• NCBI GI #: 4758284
• NCBI GeneID: 2047
• NCBI Accession #: NP_004432.1
• NCBI GenBank Nucleotide #: NM_004441.4
• UniProt Accession #: P54762; O43569; O95142; O95143; Q0VG87; A8K593
• Molecular Weight: 109,885 Da
• NCBI Official Full Name: ephrin type-B receptor 1
• NCBI Official Synonym Full Names: EPH receptor B1
• NCBI Official Symbol: EPHB1
• NCBI Official Synonym Symbols: ELK; NET; Hek6; EPHT2
• NCBI Protein Information: ephrin type-B receptor 1; EK6; EPH-like kinase 6; eph tyrosine kinase 2; soluble EPHB1 variant 1; tyrosine-protein kinase receptor EPH-2; neuronally-expressed EPH-related tyrosine kinase
• UniProt Protein Name: Ephrin type-B receptor 1
• UniProt Gene Name: EPHB1
• UniProt Synonym Gene Names: ELK; EPHT2; HEK6; NET; EK6; hEK6; NET
• UniProt Entry Name: EPHB1_HUMAN