FCRN/FCGRT&B2M active protein

Recombinant human FCRN/FCGRT&B2M protein

Cat. Num. AAA48991

• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: FCRN/FCGRT&B2M; N/A; Recombinant human FCRN/FCGRT&B2M protein; FCGRT; IgG Fc fragment receptor transporter alpha chain; IgG receptor FcRn large subunit p51 isoform1; Fcgrt; FcRn; Fc gamma receptor and transporter; FcgammaRn; Transmembrane alpha chain of the neonatal receptor; Neonatal Fc receptor; FCRN; alpha-chain; Beta-2-microglobulin; IMD43; CDABP0092; HDCMA22P; FCRN/FCGRT&B2M active protein

• Host: HEK293
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid in. Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 24-297aa (FCGRT), 21-119aa (B2M)
• Sequence: Ala24, Ile21; FCGRT>AESHLSLLYH LTAVSSPAPG TPAFWVSGWL GPQQYLSYNS LRGEAEPCGA WVWENQVSWY WEKETTDLRI KEKLFLEAFK ALGGKGPYTL QGLLGCELGP DNTSVPTAKF ALNGEEFMNF DLKQGTWGGD WPEALAISQR WQQQDKAANK ELTFLLFSCP HRLREHLERG RGNLEWKEPP SMRLKARPSS PGFSVLTCSA FSFYPPELQL RFLRNGLAAG TGQGDFGPNS DGSFHASSSL TVKSGDEHHY CCIVQHAGLA QPLRVELESP AKSS; B2M>IQRTPKIQVY SRHPAENGKS NFLNCYVSGF HPSDIEVDLL KNGERIEKVE HSDLSFSKDW SFYLLYYTEF TPTEKDEYAC RVNHVTLSQP KIVKWDRDM

• Applicable Applications for FCRN/FCGRT&B2M active protein: Bioactivity, SDS-PAGE
• Species: Human
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
• Tag: His-Tag
• Preparation and Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Related Product Information for FCRN/FCGRT&B2M active protein

FCRN/FCGRT, also known as IgG receptor FcRn large subunit p51, is an IgG Fc receptor which is similar in structure to the MHC class I molecule and also associates with beta-2-microglobulin. This protein is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis. Also, it is expessed in neutrophils and myeloid antigen presenting cells. It is present in the placenta where it transports mother's IgG to the growing fetus. In addition to binding to IgG, it has been shown to interact with human serum albumin. FCRN/FCGRT- mediated transcytosis of IgG across epithelial cells is possible because FCRN binds IgG at acidic pHbut not at neutral or higher pH. B2M, also known as beta 2-Microglobulin, is a component of MHC class I molecules, Involved in the presentation of peptide antigens to the immune system. It is a protein found on the surface of many cells and plentiful on the surface of white blood cells. B2M associates not only with the alpha chain of MHC class I molecules, but also with class I-like molecules such as CD1, MR1, the neonatal Fc receptor (FCRN/FCGRT), and Qa-1. Recombinant human FCGRT fused to His-tag at C-terminus, and B2M was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Categories/Family for FCRN/FCGRT&B2M active protein

Immunology, Cardiovascular; Proteins

References

Akilesh, S. et al. (2008) Proc. Natl. Acad. Sci. 105:967-972.
Morabito A., et al. (2009) Hum Immunol. 70:492-495.

NCBI and Uniprot Product Information

• UniProt Accession #: P55899; P61769
• Molecular Weight: 31.1kDa (FCGRT, 280aa); 11.7kDa (B2M, 99aa)
• UniProt Protein Name: IgG receptor FcRn large subunit p51
• UniProt Gene Name: FCGRT
• UniProt Synonym Gene Names: FCRN; FcRn
• UniProt Entry Name: FCGRN_HUMAN