Application Data
(Measured by its binding ability in a functional ELISA. Immobilized zebrafish EFNB2A-His at 10 ug/ml (100 ul/well) can bind human EphB4-Fc, The EC50 of human EphB4-Fc is 15.5-36.3 ng/ml.)
EFNB2A active protein
EFNB2A Protein, Danio rerio (zebrafish), Recombinant (His Tag)
Gene Names
efnb2a; efnb2; ephrin-B2a; id:ibd5072
Purity
>95% as determined by SDS-PAGE
Synonyms
EFNB2A; N/A; EFNB2A Protein, Danio rerio (zebrafish), Recombinant (His Tag); Danio rerio (zebrafish) EFNB2A/Ephrin B2a Protein (His Tag); efnb2 Protein, Danio rerio (zebrafish); EFNB2A Protein, Danio rerio (zebrafish); id:ibd5072 Protein, Danio rerio (zebrafish); EFNB2A active protein
Host
HEK293 Cells
Purity/Purification
>95% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Ala222
Species
Danio rerio (zebrafish)
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Leu 25
Tag
C-His
Protein Construction
A DNA sequence encoding the zebrafish EFNB2A (O73874)(Met 1-Ala222) was expressed with a polyhistidine tag at the C-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Activity
Measured by its binding ability in a functional ELISA. Immobilized zebrafish EFNB2A-His at 10ug/ml (100uL/well) can bind human EphB4-Fc. The EC50 of human EphB4-Fc is 15.5-36.3ng/mL.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized zebrafish EFNB2A-His at 10 ug/ml (100 ul/well) can bind human EphB4-Fc, The EC50 of human EphB4-Fc is 15.5-36.3 ng/ml.)
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized zebrafish EFNB2A-His at 10 ug/ml (100 ul/well) can bind human EphB4-Fc, The EC50 of human EphB4-Fc is 15.5-36.3 ng/ml.)
SDS-PAGE
SDS-PAGE
Related Product Information for EFNB2A active protein
Background: Efnb2a, also known as ephrin B2a, belongs to the ephrin family. Members of this family are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Efnb2a binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Efnb2a, together with ephb4 may play a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration.
References
Shaw KM. et al., 2006, Dev Dyn. 235 (7): 1753-60. Kemp HA. et al., 2009, Dev Biol. 327 (2): 313-26. Patten SA. et al., 2012, PLoS One. 7 (2): e31650.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36,724 Da
NCBI Official Full Name
ephrin-B2a
NCBI Official Synonym Full Names
ephrin B2a
NCBI Official Symbol
efnb2a
NCBI Official Synonym Symbols
efnb2; ephrin-B2a; id:ibd5072
NCBI Protein Information
ephrin-B2a
UniProt Protein Name
Ephrin-B2a
UniProt Gene Name
efnb2a
UniProt Synonym Gene Names
efnb2
UniProt Entry Name
EFNB2_DANRE
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Product Notes
The EFNB2A efnb2a (Catalog #AAA258249) is an Active Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Ala22 2. It is sometimes possible for the material contained within the vial of "EFNB2A, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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