Application Data
(Measured by its binding ability in a functional ELISA. Immobilized mouse EPHA1-His at 10 ug/ml (100 ul/well) can bind mouse EFNA1-Fc, The EC50 of mouse EFNA1-Fc is 21.3-49.8 ng/ml.)
EPHA1 active protein
EphA1 Protein, Mouse, Recombinant (His Tag)
Gene Names
Epha1; Eph; Esk; AL033318; 5730453L17Rik
Purity
>97% as determined by SDS-PAGE
Synonyms
EphA1; N/A; EphA1 Protein, Mouse, Recombinant (His Tag); Mouse EphA1/EPH receptor A1 Protein (His Tag); 5730453L17Rik Protein, Mouse; AL033318 Protein, Mouse; Eph Protein, Mouse; Esk Protein, Mouse; EPH receptor A1; EPHA1 active protein
Host
HEK293 Cells
Purity/Purification
>97% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Glu548
Species
Mouse
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Arg 25
Tag
C-His
Protein Construction
A DNA sequence encoding the extracellular domain of mouse EPHA1 (Q60750)(Met 1-Glu 548) was expressed, with a C-terminal polyhistidine tag.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Activity
Measured by its binding ability in a functional ELISA. Immobilized mouse EPHA1-His at 10ug/ml (100uL/well) can bind mouse EFNA1-Fc. The EC50 of mouse EFNA1-Fc is 21.3-49.8ng/mL.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized mouse EPHA1-His at 10 ug/ml (100 ul/well) can bind mouse EFNA1-Fc, The EC50 of mouse EFNA1-Fc is 21.3-49.8 ng/ml.)
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized mouse EPHA1-His at 10 ug/ml (100 ul/well) can bind mouse EFNA1-Fc, The EC50 of mouse EFNA1-Fc is 21.3-49.8 ng/ml.)
SDS-PAGE
SDS-PAGE
Related Product Information for EPHA1 active protein
Background: EPHA1 or EPH receptor A1 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. An important role of Eph receptors and their ligands ephrins is to mediate cell-contact-dependent repulsion. Eph receptors and ephrins also act at boundaries to channel neuronal growth cones along specific pathways, restrict the migration of neural crest cells, and via bidirectional signaling prevent intermingling between hindbrain segments. Eph receptors and ephrins can also trigger an adhesive response of endothelial cells and are required for the remodeling of blood vessels. Eph receptors and ephrins have emerged as key regulators of the repulsion and adhesion of cells that underlie the establishment, maintainence, and remodeling of patterns of cellular organization. The ephrins and Eph receptors are implicated as positional labels that may guide the development of neural topographic maps.
Product Categories/Family for EPHA1 active protein
References
Flanagan JG, et al. (1998) THE EPHRINS AND EPH RECEPTORS IN NEURAL DEVELOPMENT. Annual Review of Neuroscience. 21: 309-45.Wilkinson DG (2000) Eph receptors and ephrins: Regulators of guidance and assembly. International Review of Cytology. 196: 177-244.Zhou R. (1998) The Eph family receptors and ligands. Pharmacol. 77 (3): 151-81.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
108,578 Da
NCBI Official Full Name
ephrin type-A receptor 1
NCBI Official Synonym Full Names
Eph receptor A1
NCBI Official Symbol
Epha1
NCBI Official Synonym Symbols
Eph; Esk; AL033318; 5730453L17Rik
NCBI Protein Information
ephrin type-A receptor 1; mEpha1; embryonic stem cell kinase; tyrosine-protein kinase receptor ESK
UniProt Protein Name
Ephrin type-A receptor 1
UniProt Gene Name
Epha1
UniProt Synonym Gene Names
Esk; mEpha1
UniProt Entry Name
EPHA1_MOUSE
Similar Products
Product Notes
The EPHA1 epha1 (Catalog #AAA258209) is an Active Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Glu54 8. It is sometimes possible for the material contained within the vial of "EphA1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.