Application Data
(Measured by its ability to inhibit mouse FGF acidic (aFGF/FGF1) dependent proliferation of Balb/C 3T3 mouse fibroblasts. The ED50 for this effect is typically 6-25 ng/mL in the presence of 0.5 ng/mL of mouse FGF acidic (aFGF/FGF1).)
FGFR1 active protein
FGFR1 Protein, Mouse, Recombinant (hFc Tag)
Gene Names
Fgfr1; FLG; MFR; Eask; Hspy; Flt-2; c-fgr; Fgfr-1; AW208770; bFGF-R-1
Purity
>97% as determined by SDS-PAGE
Synonyms
FGFR1; N/A; FGFR1 Protein, Mouse, Recombinant (hFc Tag); Mouse FGFR1/CD331 Protein (Fc Tag); AW208770 Protein, Mouse; bFGF-R-1 Protein, Mouse; c-fgr Protein, Mouse; Eask Protein, Mouse; Fgfr-1 Protein, Mouse; FLG Protein, Mouse; Flt-2 Protein, Mouse; Hspy Protein, Mouse; MFR Protein, Mouse; fibroblast growth factor receptor 1; FGFR1 active protein
Host
HEK293 Cells
Purity/Purification
>97% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Glu376
Species
Mouse
Activity
Measured by its ability to inhibit mouse FGF acidic (aFGF/FGF1) dependent proliferation of Balb/C 3T3 mouse fibroblasts. The ED50 for this effect is typically 6-25ng/mL in the presence of 0.5ng/mL of mouse FGF acidic (aFGF/FGF1).
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Arg 22
Tag
C-human IgG1-Fc
Protein Construction
A DNA sequence encoding the extracellular domain (Met 1-Glu 376) of mouse FGFR1 isoform 1 (P16092-1) was as fused with the Fc region of human IgG1 at the C-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
Application Data
(Measured by its ability to inhibit mouse FGF acidic (aFGF/FGF1) dependent proliferation of Balb/C 3T3 mouse fibroblasts. The ED50 for this effect is typically 6-25 ng/mL in the presence of 0.5 ng/mL of mouse FGF acidic (aFGF/FGF1).)
Application Data
(Measured by its ability to inhibit mouse FGF acidic (aFGF/FGF1) dependent proliferation of Balb/C 3T3 mouse fibroblasts. The ED50 for this effect is typically 6-25 ng/mL in the presence of 0.5 ng/mL of mouse FGF acidic (aFGF/FGF1).)
SDS-PAGE
SDS-PAGE
Related Product Information for FGFR1 active protein
Background: FGFR1, also known as CD331, belongs to the fibroblast growth factor receptor subfamily where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. Fibroblast growth factors (FGFs)(FGF1 - 10 and 16 - 23) are mitogenic signaling molecules that have roles in angiogenesis, wound healing, cell migration, neural outgrowth and embryonic development. FGFs bind heparan sulfate glycosaminoglycans, which facilitates dimerization (activation) of FGF receptors. FGFR1 is a full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of FGFR1 interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction. CD331 can be detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells. Defects in FGFR1 are a cause of Pfeiffer syndrome, idiopathic hypogonadotropic hypogonadism, Kallmann syndrome type 2, osteoglophonic dysplasia and trigonocephaly non-syndromic.
Product Categories/Family for FGFR1 active protein
References
Schlessinger J, et al. (2000) Crystal structure of a ternary FGF-FGFR-heparin complex reveals a dual role for heparin in FGFR binding and dimerization. Mol Cell. 6(3):743-50.
Doda© C, et al. (2007) Novel FGFR1 sequence variants in Kallmann syndrome, and genetic evidence that the FGFR1c isoform is required in olfactory bulb and palate morphogenesis. Hum Mutat. 28(1): 97-8.
Kim HG, et al. (2005) Hypogonadotropic hypogonadism and cleft lip and palate caused by a balanced translocation producing haploinsufficiency for FGFR1. J Med Genet. 42(8):666-72.
Doda© C, et al. (2007) Novel FGFR1 sequence variants in Kallmann syndrome, and genetic evidence that the FGFR1c isoform is required in olfactory bulb and palate morphogenesis. Hum Mutat. 28(1): 97-8.
Kim HG, et al. (2005) Hypogonadotropic hypogonadism and cleft lip and palate caused by a balanced translocation producing haploinsufficiency for FGFR1. J Med Genet. 42(8):666-72.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
91,669 Da
NCBI Official Full Name
fibroblast growth factor receptor 1 isoform 2
NCBI Official Synonym Full Names
fibroblast growth factor receptor 1
NCBI Official Symbol
Fgfr1
NCBI Official Synonym Symbols
FLG; MFR; Eask; Hspy; Flt-2; c-fgr; Fgfr-1; AW208770; bFGF-R-1
NCBI Protein Information
fibroblast growth factor receptor 1
UniProt Protein Name
Fibroblast growth factor receptor 1
UniProt Gene Name
Fgfr1
UniProt Synonym Gene Names
Flg; FGFR-1; bFGF-R-1
UniProt Entry Name
FGFR1_MOUSE
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Product Notes
The FGFR1 fgfr1 (Catalog #AAA258140) is an Active Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Glu37 6. It is sometimes possible for the material contained within the vial of "FGFR1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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