Rabbit anti-Human FGFR1 Polyclonal Antibody | anti-FGFR1 antibody

FGFR1 Antibody (N-term)

Cat. Num. AAA284197

• Gene Names: FGFR1; CEK; FLG; HH2; OGD; FLT2; KAL2; BFGFR; CD331; FGFBR; FLT-2; HBGFR; N-SAM; FGFR-1; HRTFDS; bFGF-R-1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Functional Assay
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: FGFR1, Antibody; FGFR1 Antibody (N-term); Fibroblast growth factor receptor 1; FGFR-1; Basic fibroblast growth factor receptor 1; BFGFR; bFGF-R-1; Fms-like tyrosine kinase 2; FLT-2; N-sam; Proto-oncogene c-Fgr; CD331; FGFR1; CEK; FGFBR; FLG; FLT2; HBGFR; anti-FGFR1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This FGFR1 antibody is generated from rabbits immunized with a His fusion protein from human FGFR1.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2mg/ml (varies by lot)
• Sequence Length: 820

• Applicable Applications for anti-FGFR1 antibody: IHC (Immunohistochemistry), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry)
• Antigen Type: Recombinant Protein
• Function: Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2. In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation.
• Cellular Location: Cell membrane; Single-pass type I membrane protein. Nucleus. Cytoplasm, cytosol. Cytoplasmic vesicle Note=After ligand binding, both receptor and ligand are rapidly internalized. Can translocate to the nucleus after internalization, or by translocation from the endoplasmic reticulum or Golgi apparatus to the cytosol, and from there to the nucleus
• Tissue Location: Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells.
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(FGFR1 Antibody FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(FGFR1 Antibody IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the FGFR1 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of FGFR1 Antibody in mouse liver tissue lysates (35ug/lane). FGFR1 (arrow) was detected using the purified Pab.)

Related Product Information for anti-FGFR1 antibody

FGFR1 is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction.

Product Categories/Family for anti-FGFR1 antibody

Cancer; Cardiovascular; Neuroscience; Signal transduction

References

Jiao, J., et al., Arch. Biochem. Biophys. 410(2):187-200 (2003). Fu, L., et al., J. Comp. Neurol. 462(2):265-273 (2003). Lundin, L., et al., Exp. Cell Res. 287(1):190-198 (2003). Kiselyov, V.V., et al., Structure (Camb.) 11(6):691-701 (2003). Baumann, H., et al., J. Biol. Chem. 278(18):16198-16208 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 291327489
• NCBI GeneID: 2260
• NCBI Accession #: NP_001167534.1; NP_001167535.1; NP_001167536.1; NP_001167537.1; NP_001167538.1; NP_056934.2; NP_075593.1; NP_075594.1; NP_075598.2
• NCBI GenBank Nucleotide #: NM_001174063.1
• UniProt Accession #: P11362; P17049; Q02063; Q02065; Q14306; Q14307; Q53H63; Q59H40; Q5BJG2; A8K6T9; A8K8V5; C1KBH8
• Molecular Weight: 91868
• NCBI Official Full Name: fibroblast growth factor receptor 1 isoform 10
• NCBI Official Synonym Full Names: fibroblast growth factor receptor 1
• NCBI Official Symbol: FGFR1
• NCBI Official Synonym Symbols: CEK; FLG; HH2; OGD; FLT2; KAL2; BFGFR; CD331; FGFBR; FLT-2; HBGFR; N-SAM; FGFR-1; HRTFDS; bFGF-R-1
• NCBI Protein Information: fibroblast growth factor receptor 1
• UniProt Protein Name: Fibroblast growth factor receptor 1
• UniProt Gene Name: FGFR1
• UniProt Synonym Gene Names: BFGFR; CEK; FGFBR; FLG; FLT2; HBGFR; FGFR-1; BFGFR; bFGF-R-1; FLT-2
• UniProt Entry Name: FGFR1_HUMAN