Application Data
(Measured by its ability to inhibit TGFbeta1 activity on Mv-1-lu mink lung epithelial cells. The ED50 for this effect is typically 1-4 ng/ml.)
HGF active protein
HGF Protein, Mouse, Recombinant
Gene Names
Hgf; SF; NK1; NK2; HGF/SF; SF/HGF; C230052L06Rik
Purity
>95% as determined by SDS-PAGE
Synonyms
HGF; N/A; HGF Protein, Mouse, Recombinant; Mouse HGF/Hepatocyte Growth Factor Protein; C230052L06Rik Protein, Mouse; HGF/SF Protein, Mouse; NK1 Protein, Mouse; NK2 Protein, Mouse; SF Protein, Mouse; SF/HGF Protein, Mouse; hepatocyte growth factor (hepapoietin A; scatter factor); HGF active protein
Host
HEK293 Cells
Purity/Purification
>95% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Leu728
Species
Mouse
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Gln 33
Tag
Native
Protein Construction
A DNA sequence encoding the mouse HGF (Q08048-1)(Met1-Leu728) was expressed and purified.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Activity
1. Immobilized mouse HGF at 10ug/ml (100uL/well) can bind mouse MET-Fc, EC50 of mouse MET-Fc is 0.05-0.12ug/ml.
2. Measured by its ability to inhibit TGFbeta1 activity on Mv-1-lu mink lung epithelial cells. The ED50 for this effect is typically 1-4ng/mL.
2. Measured by its ability to inhibit TGFbeta1 activity on Mv-1-lu mink lung epithelial cells. The ED50 for this effect is typically 1-4ng/mL.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
Application Data
(Measured by its ability to inhibit TGFbeta1 activity on Mv-1-lu mink lung epithelial cells. The ED50 for this effect is typically 1-4 ng/ml.)
Application Data
(Measured by its ability to inhibit TGFbeta1 activity on Mv-1-lu mink lung epithelial cells. The ED50 for this effect is typically 1-4 ng/ml.)
SDS-PAGE
SDS-PAGE
Related Product Information for HGF active protein
Background: Hepatocyte growth factor, also known as HGF, contains 4 kringle domains, 1 PAN domain, and 1 peptidase S1 domain. It belongs to the peptidase S1 family, plasminogen subfamily. The hepatocyte growth factor is secreted by mesenchymal cells as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor, and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility and matrix invasion give it a central role in angiogenesis, tumorogenesis, and tissue regeneration. HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. HGF has no detectable protease activity. Defects in hepatocyte growth factor are the cause of deafness autosomal recessive type 39. A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
Product Categories/Family for HGF active protein
References
Naldini L, et al. (1991) Scatter factor and hepatocyte growth factor are indistinguishable ligands for the MET receptor. EMBO J. 10(10):2867-78.
Comoglio, et al. (1993) Structure, biosynthesis and biochemical properties of the HGF receptor in normal and malignant cells. 65:131-65.
Hahn W, et al. (2011) Enhanced cardioprotective effects by coexpression of two isoforms of hepatocyte growth factor from naked plasmid DNA in a rat ischemic heart disease model. The Journal of Gene Medicine. 13(10):549-55.
Bottaro DP, et al. (1991) Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product. Science. 251(4995):802-4.
Comoglio, et al. (1993) Structure, biosynthesis and biochemical properties of the HGF receptor in normal and malignant cells. 65:131-65.
Hahn W, et al. (2011) Enhanced cardioprotective effects by coexpression of two isoforms of hepatocyte growth factor from naked plasmid DNA in a rat ischemic heart disease model. The Journal of Gene Medicine. 13(10):549-55.
Bottaro DP, et al. (1991) Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product. Science. 251(4995):802-4.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,308 Da
NCBI Official Full Name
hepatocyte growth factor isoform 1 preproprotein
NCBI Official Synonym Full Names
hepatocyte growth factor
NCBI Official Symbol
Hgf
NCBI Official Synonym Symbols
SF; NK1; NK2; HGF/SF; SF/HGF; C230052L06Rik
NCBI Protein Information
hepatocyte growth factor
UniProt Protein Name
Hepatocyte growth factor
UniProt Gene Name
Hgf
UniProt Synonym Gene Names
SF
UniProt Entry Name
HGF_MOUSE
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Product Notes
The HGF hgf (Catalog #AAA258122) is an Active Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Leu72 8. It is sometimes possible for the material contained within the vial of "HGF, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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