WB (Western Blot)
(3T3-L1 preadipocytes were incubated with 1 pg/mL-0.1ug/mL of Recombinant Human Irisin for 12 minutes. The cells were lysed and Western blots were performed with anti-phospho p38 MAPK and total p38 MAPK antibodies.)
Irisin Active Protein | FNDC5 active protein
Recombinant Human/Mouse/Rat Irisin Protein
Gene Names
FNDC5; FRCP2; irisin
Purity
>95% by SDS-PAGE.
Synonyms
Irisin; N/A; Recombinant Human/Mouse/Rat Irisin Protein; fibronectin type III domain containing 5; fibronectin type III domain-containing protein 5; FNDC5; FRCP2; FRCP2Fibronectin type III repeat-containing protein 2; FNDC5 active protein
Host
HEK293 cells
Purity/Purification
>95% by SDS-PAGE.
Form/Format
Lyophilized from a 0.22 um filtered solution of PBS, pH 7.4.
Species
Human
Species Reactivity/CrossReactivity
Human, Mouse, Rat
Endotoxin
<0.1EU/ug of the protein by LAL method.
Tag
C-His
Reconstitution
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
BioActivity
Measured by its ability to induce p38 MAPK activation in 3T3 L1 mouse embryonic fibroblast adipose-like cells. 0.1 ?g/mL of Recombinant Human Irisin can effectively induce p38 MAPK activation.
Preparation and Storage
Store the lyophilized protein at -20°C to -80°C for long term.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. Avoid repeated freeze/thaw cycles.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. Avoid repeated freeze/thaw cycles.
WB (Western Blot)
(3T3-L1 preadipocytes were incubated with 1 pg/mL-0.1ug/mL of Recombinant Human Irisin for 12 minutes. The cells were lysed and Western blots were performed with anti-phospho p38 MAPK and total p38 MAPK antibodies.)
WB (Western Blot)
(3T3-L1 preadipocytes were incubated with 1 pg/mL-0.1ug/mL of Recombinant Human Irisin for 12 minutes. The cells were lysed and Western blots were performed with anti-phospho p38 MAPK and total p38 MAPK antibodies.)
Size Exclusion Chromatography-HPLC
(The purity of Human/Mouse/Rat Irisin Protein (Cat.AAA282163) was greater than 90% as determied by SEC-HPLC.)
Size Exclusion Chromatography-HPLC
(The purity of Human/Mouse/Rat Irisin Protein (Cat.AAA282163) was greater than 90% as determied by SEC-HPLC.)
SDS-PAGE
(Recombinant human Irisin Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 20-28 kDa.)
SDS-PAGE
(Recombinant human Irisin Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 20-28 kDa.)
Related Product Information for FNDC5 active protein
Recombinant Human/Mouse/Rat Irisin Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Asp32-Glu143) of human Irisin (Accession #NP_715637.2) fused with a 6×His tag at the C-terminus.
Product Categories/Family for FNDC5 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
fibronectin type III domain-containing protein 5 isoform 2 preproprotein
NCBI Official Synonym Full Names
fibronectin type III domain containing 5
NCBI Official Symbol
FNDC5
NCBI Official Synonym Symbols
FRCP2; irisin
NCBI Protein Information
fibronectin type III domain-containing protein 5
UniProt Protein Name
Fibronectin type III domain-containing protein 5
UniProt Gene Name
FNDC5
UniProt Synonym Gene Names
FRCP2
UniProt Entry Name
FNDC5_HUMAN
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Product Notes
The FNDC5 fndc5 (Catalog #AAA282163) is an Active Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Irisin, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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