KLK4 active protein

KLK4 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA27762

• Gene Names: KLK4; ARM1; EMSP; PSTS; AI2A1; EMSP1; KLK-L1; PRSS17; MGC116827; MGC116828
• Purity: >94% as determined by SDS-PAGE
• Synonyms: KLK4; KLK4 Protein; Human; Recombinant (His Tag); Human KLK-4/Kallikrein-4 Protein (His Tag); AI2A1 Protein; ARM1 Protein; EMSP Protein; EMSP1 Protein; kallikrein Protein; KLK-L1 Protein; PRSS17 Protein; PSTS Protein; kallikrein-related peptidase 4; KLK4 active protein

• Host: HEK293 Cells
• Purity/Purification: >94% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%trehalose, 5% mannitol, 0.01% Tween80.
• Sequence: Met1-Ser254

• Source: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ser 27
• Tag: His
• Protein Construction: A DNA sequence encoding the human KLK4 (NP_004908.3)(Met 1-Ser 254) was expressed, with a polyhistidine tag at the C-terminus.
• Reconstitution: It is recommended that sterile water (200ul) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV.
• Activity: Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC.. The specific activity is >250pmoles/min/ug. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)
• Preparation and Storage: Store it under sterile conditions at -20? to -80? upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.

SDS-PAGE

Related Product Information for KLK4 active protein

Description: Kallikrein-4, also known as Enamel matrix serine proteinase 1, Kallikrein- like protein 1, KLK-L1, Serine protease 17, KLK4, PRSS17 and EMSP1, is a secreted protein which belongs to thepeptidase S1 family and Kallikrein subfamily. Kallikrein-4 / KLK4 is a serine protease expressed during enamel maturation, and proteolytic processing of the enamel matrix by KLK4 is critical for proper enamel formation. Kallikrein-4 / KLK4 contains onepeptidase S1 domain. Kallikrein-4 / KLK4 is secreted by transition- and maturation-stage ameloblasts. KLK4 aggressively degrades the retained organic matrix following the termination of enamel protein secretion. Two proteases are secreted into the enamel matrix of developing teeth. The early protease is enamelysin (MMP-20). The late protease is kallikrein 4 (KLK4). The principle functions of MMP-20 and KLK4 in dental enamel formation are to facilitate the orderly replacement of organic matrix with mineral, generating an enamel layer that is harder, less porous, and unstained by retained enamel proteins. Defects in Kallikrein-4 / KLK4 are the cause of amelogenesis imperfecta hypomaturation type 2A1 (AI2A1) which is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentions.

Product Categories/Family for KLK4 active protein

Serine Proteases and Regulators

References

Nelson P.S., et al.,(1999), Molecular cloning and characterization of prostase, an androgen-regulated serine protease with prostate-restricted expression. Proc. Natl. Acad. Sci. U.S.A. 96:3114-3119.Yousef G.M., et al., (1999), Prostase/KLK-L1 is a new member of the human kallikrein gene family, is expressed in prostate and breast tissues, and is hormonally regulated.Cancer Res. 59:4252-4256.Stephenson S.A., et al.,(1999), Localization of a new prostate-specific antigen-related serine protease gene, KLK4, is evidence for an expanded human kallikrein gene family cluster on chromosome 19q13.3-13.4.J. Biol. Chem. 274:23210-23214.

NCBI and Uniprot Product Information

• NCBI GI #: 89142741
• NCBI GeneID: 9622
• NCBI Accession #: NP_004908.3
• NCBI GenBank Nucleotide #: NM_004917.3
• UniProt Accession #: Q4VB17; Q4VB18; Q5BQA0; Q96JD7; Q96JD8; Q96JD9; Q96JE0; Q96JE1; Q96JE2; Q96PT0
• Molecular Weight: The secreted recombinant human KLK4 consists of 239 amino acids and predictes a molecular mass of 25.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhKLK4 is approximately 30-35 kDa due to glycosylation
• NCBI Official Full Name: kallikrein-4 preproprotein
• NCBI Official Synonym Full Names: kallikrein-related peptidase 4
• NCBI Official Symbol: KLK4
• NCBI Official Synonym Symbols: ARM1; EMSP; PSTS; AI2A1; EMSP1; KLK-L1; PRSS17; MGC116827; MGC116828
• NCBI Protein Information: kallikrein-4; prostase; serine protease 17; kallikrein-like protein 1; androgen-regulated message 1; enamel matrix serine protease 1; enamel matrix serine proteinase 1
• UniProt Protein Name: Kallikrein-related peptidase 4
• UniProt Gene Name: KLK4
• UniProt Entry Name: Q4VB17_HUMAN