Application Data
(Recombinant Human MMP-13 induces cell migration of the A549 cells. 10 ng/mL of Recombinant Human MMP-13 can effectively induce A549 cells migration.)
MMP-13 active protein
Recombinant Human MMP-13 Protein
Gene Names
MMP13; CLG3; MANDP1
Purity
>95% by SDS-PAGE.
Synonyms
MMP-13; N/A; Recombinant Human MMP-13 Protein; CLG3; MANDP1; MDST; MMP-13 active protein
Host
HEK293 cells
Purity/Purification
>95% by SDS-PAGE.
Form/Format
Lyophilized from a 0.22um filtered solution of PBS, pH7.4.
Species
Human
Endotoxin
<0.1EU/ug of the protein by LAL method.
Tag
C-6xHis
Reconstitution
Reconstitute to a concentration of 0.1-0.5mg/mL in sterile distilled water.
BioActivity
Measured in a cell migration assay using A549 cells. 10ng/mL of Recombinant Human MMP-13 can effectively induce A549 cells migration.
Preparation and Storage
Store the lyophilized protein at -20 degree C to -80 degree C for long term.
After reconstitution, the protein solution is stable at -20 degree C for 3 months, at 2-8 degree C for up to 1 week. Avoid freeze/thaw cycles.
After reconstitution, the protein solution is stable at -20 degree C for 3 months, at 2-8 degree C for up to 1 week. Avoid freeze/thaw cycles.
Application Data
(Recombinant Human MMP-13 induces cell migration of the A549 cells. 10 ng/mL of Recombinant Human MMP-13 can effectively induce A549 cells migration.)
Application Data
(Recombinant Human MMP-13 induces cell migration of the A549 cells. 10 ng/mL of Recombinant Human MMP-13 can effectively induce A549 cells migration.)
SDS-PAGE
(Recombinant Human MMP-13 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 58 kDa.)
SDS-PAGE
(Recombinant Human MMP-13 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 58 kDa.)
Related Product Information for MMP-13 active protein
Recombinant Human MMP-13 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu20-Cys471) of human MMP-13 (Accession #NP_002418.1) fused with a 6×His tag at the C-terminus.
Background: Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases. MMP-13 (Collagenase-3) has been demonstrated to degrade a range of extracellular matrix proteins, including collagen types I, II, III, IV, IX, X and XIV, gelatin, aggrecan, perlecan and fibronectin. MMP-13 is proteolytically processed to generate the mature protease. MMP-13 is distinguished from the other human collagenases, it cleaves type II collagen more efficiently than types I and III. MMP-13 is expressed by fibroblasts, chrondrocytes and squamous epithelial cells. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis.
Background: Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases. MMP-13 (Collagenase-3) has been demonstrated to degrade a range of extracellular matrix proteins, including collagen types I, II, III, IV, IX, X and XIV, gelatin, aggrecan, perlecan and fibronectin. MMP-13 is proteolytically processed to generate the mature protease. MMP-13 is distinguished from the other human collagenases, it cleaves type II collagen more efficiently than types I and III. MMP-13 is expressed by fibroblasts, chrondrocytes and squamous epithelial cells. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis.
Product Categories/Family for MMP-13 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
collagenase 3 preproprotein
NCBI Official Synonym Full Names
matrix metallopeptidase 13 (collagenase 3)
NCBI Official Symbol
MMP13
NCBI Official Synonym Symbols
CLG3; MANDP1
NCBI Protein Information
collagenase 3; MMP-13; matrix metalloproteinase 13 (collagenase 3)
UniProt Protein Name
Collagenase 3
UniProt Gene Name
MMP13
UniProt Synonym Gene Names
MMP-13
UniProt Entry Name
MMP13_HUMAN
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Product Notes
The MMP-13 mmp13 (Catalog #AAA282161) is an Active Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "MMP-13, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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