PCSK9 active protein

PCSK9 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA258147

• Gene Names: Pcsk9; FH3; PC9; Narc1; HCHOLA3; AI415265; AI747682
• Purity: >95% as determined by SDS-PAGE
• Synonyms: PCSK9; N/A; PCSK9 Protein, Mouse, Recombinant (His Tag); Mouse PCSK9/NARC1 Protein (His Tag); AI415265 Protein, Mouse; AI747682 Protein, Mouse; FH3 Protein, Mouse; HCHOLA3 Protein, Mouse; Narc1 Protein, Mouse; PC9 Protein, Mouse; proprotein convertase subtilisin/kexin type 9; PCSK9 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 15mM Tris, 90mM NaCl, 50% Glycerol, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gln694

• Species: Mouse
• Activity: Measured by its binding ability in a functional ELISA. Immobilized mouse PCSK9 at 10ug/ml (100uL/well) can bind biotinylated recombinant human LDLR. The EC50 of biotinylated human LDLR is 0.12ug/ml.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gln 35
• Tag: C-His
• Protein Construction: A DNA sequence encoding the full length of mouse PCSK9 (NP_705793.1) precursor (Met 1-Gln 694) was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

Application Data

(Measured by its binding ability in a functional ELISA. Immobilized mouse PCSK9 at 10 ug/ml (100 ul/well) can bind biotinylated recombinant human LDLR. The EC50 of biotinylated human LDLR is 0.12 ug/ml.)

SDS-PAGE

Related Product Information for PCSK9 active protein

Background: Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.

Product Categories/Family for PCSK9 active protein

Serine Proteases and Regulators

References

Sseidah, N.G. et al., 2003, Proc. Natl. Acad. Sci. USA. 100: 928-933. Beyer, T.P. et al., 2007, J. Lipid. Res. 48: 1488-1498 Shan, L. et al., 2008, Biochem. Biophys. Res. Commun. 375: 69-73. Benjannet, S. et al., 2005, J. Biol. Chem. 279: 48865-48875. Abifadel, M. et al., 2003, Nat. Genet. 34: 154-156.

NCBI and Uniprot Product Information

• NCBI GI #: 23956352
• NCBI GeneID: 100102
• NCBI Accession #: NP_705793.1
• NCBI GenBank Nucleotide #: NM_153565.2
• UniProt Accession #: Q80W65; Q3UEH7; Q8BXW9; Q8CFT6; B1AZI4
• Molecular Weight: 74,823 Da
• NCBI Official Full Name: proprotein convertase subtilisin/kexin type 9
• NCBI Official Synonym Full Names: proprotein convertase subtilisin/kexin type 9
• NCBI Official Symbol: Pcsk9
• NCBI Official Synonym Symbols: FH3; PC9; Narc1; HCHOLA3; AI415265; AI747682
• NCBI Protein Information: proprotein convertase subtilisin/kexin type 9
• UniProt Protein Name: Proprotein convertase subtilisin/kexin type 9
• UniProt Gene Name: Pcsk9
• UniProt Synonym Gene Names: Narc1; NARC-1; PC9
• UniProt Entry Name: PCSK9_MOUSE