SDS-PAGE
TCN2 active protein
Recombinant Human TCN2 Protein (His tag)
Gene Names
TCN2; II; TC; TC2; TC-2; TCII; TC II; D22S676; D22S750
Purity
> 90 % as determined by reducing SDS-PAGE.
Synonyms
TCN2; N/A; Recombinant Human TCN2 Protein (His tag); D22S676; D22S750; II; TC; TC-2; TC2; TCII; TCN2 active protein
Host
HEK293 Cells
Purity/Purification
> 90 % as determined by reducing SDS-PAGE.
Form/Format
Lyophilized from sterile PBS, pH 7.4, 5% Trehalose, 5% Mannitol
Sequence Positions
Met 1-Trp 427
Species
Human
Sequence
Met 1-Trp 427
Tag
C-His
Bio Activity
Measured by its binding ability in a functional ELISA. Immobilized human TCN2-His at 10ug/mL (100uL/well) can bind biotinylated mouse CD320-His. The EC50 of biotinylated mouse CD320-His is 18-42 ng/mL.
Endotoxin
< 1.0 EU per ug as determined by the LAL method.
Reconstitution
It is recommended that sterile water (200ul) be added to the vial to prepare a stock solution of 0.1mg/mL.
Concentration is measured by UV-Vis.
Concentration is measured by UV-Vis.
Preparation and Storage
Ships on blue ice.
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C.
Reconstituted protein solution can be stored at 4-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C.
Reconstituted protein solution can be stored at 4-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
SDS-PAGE
SDS-PAGE
Related Product Information for TCN2 active protein
Transcobalamin II, also known as TCN2 and TC II, is a plasma protein that binds cobalamin (Cbl; vitamin B12) as it is absorbed in the terminal ileum and distributes to tissues. The circulating transcobalamin II-cobalamin complex binds to receptors on the plasma membrane of tissue cells and is then internalized by receptor- mediated endocytosis. Transcobalamin II is a non-glycolated secretory protein of molecular mass 43 kDa. Its plasma membrane receptor (TC IIR) is a heavily glycosylated protein with a monomeric molecular mass of 62 kDa. Human TCN2 gene is composed of nine exons and eight introns spanning approximately 20 kb with multiple potential transcription start sites. A number of genetic abnormalities are characterized either by a failure to express TCN2 or by synthesis of an abnormal protein. The TCN2 deficiency results in cellular cobalamin deficiency, an early onset of megaloblastic anaemia, and neurological abnormalities.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Molecular Mass: 46.7 kDa
Actual Protein Molecular Mass: 43 kDa
Actual Protein Molecular Mass: 43 kDa
NCBI Official Full Name
transcobalamin-2 isoform 1
NCBI Official Synonym Full Names
transcobalamin 2
NCBI Official Symbol
TCN2
NCBI Official Synonym Symbols
II; TC; TC2; TC-2; TCII; TC II; D22S676; D22S750
NCBI Protein Information
transcobalamin-2
UniProt Protein Name
Transcobalamin-2
UniProt Gene Name
TCN2
UniProt Synonym Gene Names
TC2; TC-2; TC II; TCII
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Product Notes
The TCN2 tcn2 (Catalog #AAA22097) is an Active Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Met 1-Trp 427. It is sometimes possible for the material contained within the vial of "TCN2, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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