Dermatophagoides pteronyssinus DERP1 Cell Lysate | DERP1 cell lysate
Dermatophagoides pteronyssinus DERP1 HEK293 Overexpression Lysate
Applications
Western Blot
Synonyms
Dermatophagoides pteronyssinus DERP1; N/A; Dermatophagoides pteronyssinus DERP1 HEK293 Overexpression Lysate; Dermatophagoides pteronyssinus DERP1 HEK293 Cell Lysate (WB positive control); DERP1 cell lysate
Host
HEK293 Cells
Form/Format
50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Applicable Applications for DERP1 cell lysate
WB (Western Blot)
Sequence information
A DNA sequence encoding the dermatophagoides pteronyssinus DERP1 (P08176) (Met1-Leu320) was expressed with a polyhistidine tag at the C-terminus.
Predicted N Terminal
Arg 19
Molecule Mass
The recombinant dermatophagoides pteronyssinus DERP1 consists 313 amino acids and predicts a molecular mass of 35.7 kDa.
Species
Dermatophagoides pteronyssinus
Preparation Method
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer
Modified RIPA
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.
Stability
Samples are stable for up to twelve months from date of receipt.
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200?L pure water and boil for 2-5 min.
3. Store the lyophilized cell lysate at 4 degree C. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80 degree C.
2. Re-dissolve the pellet using 200?L pure water and boil for 2-5 min.
3. Store the lyophilized cell lysate at 4 degree C. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80 degree C.
Storage Buffer
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Preparation and Storage
Store at 4 degreee C. After re-dissolution, aliquot and store at -80 degree C.
Related Product Information for DERP1 cell lysate
Human Cells that Dermatophagoides pteronyssinus DERP1 Heterodimer transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
NCBI and Uniprot Product Information
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Product Notes
The DERP1 derp1 (Catalog #AAA257385) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Dermatophagoides pteronyssinus DERP1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the DERP1 derp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dermatophagoides pteronyssinus DERP1, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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